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However, most of the predicted lipoproteins are of unknown function, and only a few proteins are known to be essential for the persistence and virulence of the pathogen. One such protein is the periplasmic BB0323, which is indispensable for <jats:italic>B. burgdorferi<\/jats:italic> to cause Lyme disease and the function of which is associated with cell fission and outer membrane integrity. After expression and transport to the periplasm, BB0323 is cleaved into C-terminal and N-terminal domains by the periplasmic serine protease BB0104. The resulting N-terminal domain is sufficient to ensure the survival of <jats:italic>B. burgdorferi<\/jats:italic> throughout the mouse\u2013tick infection cycle. The crystal structure of the N-terminal domain of BB0323 was determined at 2.35\u2005\u00c5 resolution. The overall fold of the protein belongs to the spectrin superfamily, with the characteristic interconnected triple-helical bundles known as spectrin repeats that function as linkers between different cell components in other organisms. Overall, the reported three-dimensional structure of the N-terminal domain of BB0323 not only reveals the molecular details of a protein that is essential for <jats:italic>B. burgdorferi<\/jats:italic> membrane integrity, cell fission and infectivity, but also suggests that spectrin repeats in bacteria are not limited to the EzrA proteins.<\/jats:p>","DOI":"10.1107\/s2059798319010751","type":"journal-article","created":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T16:22:18Z","timestamp":1566490938000},"page":"825-830","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1107","volume":"75","author":[{"given":"Kalvis","family":"Brangulis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inara","family":"Akopjana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andris","family":"Kazaks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaspars","family":"Tars","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"jb5012_bb1","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/S0969-2126(00)80062-3","volume":"7","author":"Abergel","year":"1999","journal-title":"Structure"},{"key":"jb5012_bb2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1107\/S0907444900019739","volume":"57","author":"Abergel","year":"2001","journal-title":"Acta Cryst. 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