{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:31:13Z","timestamp":1790667073771,"version":"4.1.0"},"reference-count":40,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"name":"National Key R&D Program","award":["2020YFA0907900"],"award-info":[{"award-number":["2020YFA0907900"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070040"],"award-info":[{"award-number":["32070040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2022,5,2]]},"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:p>\n                    Transient protein\u2013protein interactions between\n                    <jats:italic>cis<\/jats:italic>\n                    -acting acyltransferase (AT) and acyl carrier protein (ACP) domains are critical for the catalysis and processivity of modular polyketide synthases (mPKSs), but are challenging for structural characterization due to the intrinsically weak binding affinity. Here, a stable complex of\n                    <jats:italic>cis<\/jats:italic>\n                    -acting AT and ACP domains from the ninth module of the salinomycin mPKS was obtained using a maleimide cross-linker and the structure of the complex was determined at 2.6\u2005\u00c5 resolution. The crystal structure shows that the AT in combination with the ketosynthase (KS)-to-AT linker forms a C-shaped architecture to embrace the ACP. The large hydrolase subdomain of the AT serves as a major binding platform for the ACP, while the small ferredoxin-like subdomain of the AT and the KS-to-AT linker cooperate with each other to constrain binding of the ACP. The importance of interface residues in\n                    <jats:italic>cis<\/jats:italic>\n                    -acting AT\u2013ACP interactions was confirmed by mutagenesis assays. The interaction mode observed in the\n                    <jats:italic>cis<\/jats:italic>\n                    -acting AT\u2013ACP complex is completely different from those observed in\n                    <jats:italic>trans<\/jats:italic>\n                    -acting AT\u2013ACP complexes, where the ACP primarily contacts the small domain of the AT. The complex structure provides detailed mechanistic insights into AT\u2013ACP recognition in\n                    <jats:italic>cis<\/jats:italic>\n                    -AT mPKSs.\n                  <\/jats:p>","DOI":"10.1107\/s2059798322004612","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T11:17:52Z","timestamp":1653477472000},"page":"779-791","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":15,"title":["Structural visualization of transient interactions between the\n                    <i>cis<\/i>\n                    -acting acyltransferase and acyl carrier protein of the salinomycin modular polyketide synthase"],"prefix":"10.1107","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9709-8849","authenticated-orcid":true,"given":"Y.","family":"Feng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Deng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Bai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"jb5038_bb1","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1110\/ps.073011407","volume":"16","author":"Alekseyev","year":"2007","journal-title":"Protein Sci."},{"key":"jb5038_bb2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1126\/science.abi8532","volume":"374","author":"Bagde","year":"2021","journal-title":"Science"},{"key":"jb5038_bb3","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/s42003-021-01838-3","volume":"4","author":"Bartholow","year":"2021","journal-title":"Commun. 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Feng\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}