{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:52:39Z","timestamp":1790506359217,"version":"4.1.0"},"reference-count":48,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770803"],"award-info":[{"award-number":["31770803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81861138047"],"award-info":[{"award-number":["81861138047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M682067"],"award-info":[{"award-number":["2020M682067"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2022,7,14]]},"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:p>\n                    The pathogen\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , which is the causative agent of Legionnaires' disease, secrets hundreds of effectors into host cells via its Dot\/Icm secretion system to subvert host-cell pathways during pathogenesis. VipF, a conserved core effector among\n                    <jats:italic>Legionella<\/jats:italic>\n                    species, is a putative acetyltransferase, but its structure and catalytic mechanism remain unknown. Here, three crystal structures of VipF in complex with its cofactor acetyl-CoA and\/or a substrate are reported. The two GNAT-like domains of VipF are connected as two wings by two \u03b2-strands to form a U-shape. Both domains bind acetyl-CoA or CoA, but only in the C-terminal domain does the molecule extend to the bottom of the U-shaped groove as required for an active transferase reaction; the molecule in the N-terminal domain folds back on itself. Interestingly, when chloramphenicol, a putative substrate, binds in the pocket of the central U-shaped groove adjacent to the N-terminal domain, VipF remains in an open conformation. Moreover, mutations in the central U-shaped groove, including Glu129 and Asp251, largely impaired the acetyltransferase activity of VipF, suggesting a unique enzymatic mechanism for the\n                    <jats:italic>Legionella<\/jats:italic>\n                    effector VipF.\n                  <\/jats:p>","DOI":"10.1107\/s2059798322007318","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T10:42:46Z","timestamp":1660041766000},"page":"1110-1119","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":5,"title":["Structural basis for the acetylation mechanism of the\n                    <i>Legionella<\/i>\n                    effector VipF"],"prefix":"10.1107","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0660-2841","authenticated-orcid":true,"given":"Tao-Tao","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory for Cellular Stress Biology, School of Life Sciences and Faculty of Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Lin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory for Cellular Stress Biology, School of Life Sciences and Faculty of Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory for Cellular Stress Biology, School of Life Sciences and Faculty of Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidong","family":"Han","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory for Cellular Stress Biology, School of Life Sciences and Faculty of Medical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"dw5230_bb2","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/bi602513x","volume":"46","author":"Berndsen","year":"2007","journal-title":"Biochemistry"},{"key":"dw5230_bb3","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1038\/4202","volume":"5","author":"Bhatnagar","year":"1998","journal-title":"Nat. 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