{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:14:03Z","timestamp":1785406443593,"version":"3.56.0"},"reference-count":43,"publisher":"International Union of Crystallography (IUCr)","issue":"7","license":[{"start":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T00:00:00Z","timestamp":1686873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T00:00:00Z","timestamp":1686873600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2023,5,14]]},"published-print":{"date-parts":[[2023,7,1]]},"abstract":"<jats:p>\n                    5-Nitrosalicylate 1,2-dioxygenase (5NSDO) is an iron(II)-dependent dioxygenase involved in the aerobic degradation of 5-nitroanthranilic acid by the bacterium\n                    <jats:italic>Bradyrhizobium<\/jats:italic>\n                    sp. It catalyzes the opening of the 5-nitrosalicylate aromatic ring, a key step in the degradation pathway. Besides 5-nitrosalicylate, the enzyme is also active towards 5-chlorosalicylate. The X-ray crystallographic structure of the enzyme was solved at 2.1\u2005\u00c5 resolution by molecular replacement using a model from the AI program\n                    <jats:italic>AlphaFold<\/jats:italic>\n                    . The enzyme crystallized in the monoclinic space group\n                    <jats:italic>P<\/jats:italic>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    , with unit-cell parameters\n                    <jats:italic>a<\/jats:italic>\n                    \u00a0=\u00a050.42,\n                    <jats:italic>b<\/jats:italic>\n                    = 143.17,\n                    <jats:italic>c<\/jats:italic>\n                    = 60.07\u2005\u00c5, \u03b2 = 107.3\u00b0. 5NSDO belongs to the third class of ring-cleaving dioxygenases. Members of this family convert\n                    <jats:italic>para<\/jats:italic>\n                    -diols or hydroxylated aromatic carboxylic acids and belong to the cupin superfamily, which is one of the most functionally diverse protein classes and is named on the basis of a conserved \u03b2-barrel fold. 5NSDO is a tetramer composed of four identical subunits, each folded as a monocupin domain. The iron(II) ion in the enzyme active site is coordinated by His96, His98 and His136 and three water molecules with a distorted octahedral geometry. The residues in the active site are poorly conserved compared with other dioxygenases of the third class, such as gentisate 1,2-dioxygenase and salicylate 1,2-dioxygenase. Comparison with these other representatives of the same class and docking of the substrate into the active site of 5NSDO allowed the identification of residues which are crucial for the catalytic mechanism and enzyme selectivity.\n                  <\/jats:p>","DOI":"10.1107\/s2059798323004199","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T12:34:55Z","timestamp":1685450095000},"page":"632-640","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of the monocupin ring-cleaving dioxygenase 5-nitrosalicylate 1,2-dioxygenase from\n                    <i>Bradyrhizobium<\/i>\n                    sp."],"prefix":"10.1107","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7511-3136","authenticated-orcid":true,"given":"Erik","family":"Eppinger","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04vnq7t77","id-type":"ROR","asserted-by":"publisher"}],"department":["Institut f\u00fcr Mikrobiologie"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Stolz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04vnq7t77","id-type":"ROR","asserted-by":"publisher"}],"department":["Institut f\u00fcr Mikrobiologie"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Ferraroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2023,6,16]]},"reference":[{"key":"nz5013_bb1","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1111\/j.1365-2958.2006.05334.x","volume":"61","author":"Adams","year":"2006","journal-title":"Mol. 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