{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:20:22Z","timestamp":1785410422904,"version":"3.56.0"},"reference-count":37,"publisher":"International Union of Crystallography (IUCr)","issue":"8","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100000266","name":"UK Research and Innovation, Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/T007338\/1"],"award-info":[{"award-number":["EP\/T007338\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2023,5,22]]},"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:p>\n                    Siderophore-binding proteins from two thermophilic bacteria,\n                    <jats:italic>Geobacillus stearothermophilus<\/jats:italic>\n                    and\n                    <jats:italic>Parageobacillus thermoglucosidasius<\/jats:italic>\n                    , were identified from a search of sequence databases, cloned and overexpressed. They are homologues of the well characterized protein\n                    <jats:italic>Cj<\/jats:italic>\n                    CeuE from\n                    <jats:italic>Campylobacter jejuni<\/jats:italic>\n                    . The iron-binding histidine and tyrosine residues are conserved in both thermophiles. Crystal structures were determined of the apo proteins and of their complexes with iron(III)-azotochelin and its analogue iron(III)-5-LICAM. The thermostability of both homologues was shown to be about 20\u00b0C higher than that of\n                    <jats:italic>Cj<\/jats:italic>\n                    CeuE. Similarly, the tolerance of the homologues to the organic solvent dimethylformamide (DMF) was enhanced, as reflected by the respective binding constants for these ligands measured in aqueous buffer at pH 7.5 in the absence and presence of 10% and 20% DMF. Consequently, these thermophilic homologues offer advantages in the development of artificial metalloenzymes using the CeuE family.\n                  <\/jats:p>","DOI":"10.1107\/s2059798323004473","type":"journal-article","created":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T13:47:28Z","timestamp":1686059248000},"page":"694-705","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":5,"title":["Thermostable homologues of the periplasmic siderophore-binding protein CeuE from\n                    <i>Geobacillus stearothermophilus<\/i>\n                    and\n                    <i>Parageobacillus thermoglucosidasius<\/i>"],"prefix":"10.1107","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-3043","authenticated-orcid":true,"given":"Elena V.","family":"Blagova","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Laboratory, Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8269-850X","authenticated-orcid":true,"given":"Alex H.","family":"Miller","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0639-8921","authenticated-orcid":true,"given":"Megan","family":"Bennett","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Laboratory, Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-1323","authenticated-orcid":true,"given":"Rosalind L.","family":"Booth","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Laboratory, Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8968-9814","authenticated-orcid":true,"given":"Eleanor J.","family":"Dodson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Laboratory, Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6214-2459","authenticated-orcid":true,"given":"Anne-Kathrin","family":"Duhme-Klair","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3581-2194","authenticated-orcid":true,"given":"Keith S.","family":"Wilson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04m01e293","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Laboratory, Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"rr5232_bb39","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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Blagova\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}