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Antifungal therapy for\n                    <jats:italic>C.\u00a0auris<\/jats:italic>\n                    infections is currently limited due to widespread resistance to fluconazole and amphotericin B and increasing resistance to the front-line drug echinocandin. Therefore, new treatments are urgently required to combat this pathogen. Dihydrofolate reductase (DHFR) has been validated as a potential drug target for\n                    <jats:italic>Candida<\/jats:italic>\n                    species, although no structure of the\n                    <jats:italic>C. auris<\/jats:italic>\n                    enzyme (CauDHFR) has been reported. Here, crystal structures of CauDHFR are reported as an apoenzyme, as a holoenzyme and in two ternary complexes with pyrimethamine and cycloguanil, which are common antifolates, at near-atomic resolution. Preliminary biochemical and biophysical assays and antifungal susceptibility testing with a variety of classical antifolates were also performed, highlighting the enzyme-inhibition rates and the inhibition of yeast growth. These structural and functional data might provide the basis for a novel drug-discovery campaign against this global threat.\n                  <\/jats:p>","DOI":"10.1107\/s2059798323004709","type":"journal-article","created":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T12:34:31Z","timestamp":1687437271000},"page":"735-745","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of dihydrofolate reductase from the emerging pathogenic fungus\n                    <i>Candida auris<\/i>"],"prefix":"10.1107","volume":"79","author":[{"given":"Tim","family":"Kirkman","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01a77tt86","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Sketcher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01a77tt86","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinicius","family":"de Morais Barroso","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Microbiology, Institute of Biomedical Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Ishida","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Microbiology, Institute of Biomedical Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Tosin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01a77tt86","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio Vinicius Bertacine","family":"Dias","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01a77tt86","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"ji5031_bb1","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1107\/S2059798318006551","volume":"74","author":"Afonine","year":"2018","journal-title":"Acta Cryst. 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