{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:56:00Z","timestamp":1790362560502,"version":"4.1.0"},"reference-count":38,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100011658","name":"French Infrastructure for Integrated Structural Biology","doi-asserted-by":"publisher","award":["ANR-10-INSB-05-01"],"award-info":[{"award-number":["ANR-10-INSB-05-01"]}],"id":[{"id":"10.13039\/501100011658","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["FRM aide aux \u00e9quipes"],"award-info":[{"award-number":["FRM aide aux \u00e9quipes"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-11-BSV8-0019"],"award-info":[{"award-number":["ANR-11-BSV8-0019"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007356","name":"Fondation M\u00e9diterran\u00e9e Infection","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007356","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2024,1,5]]},"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:p>\n                  <jats:italic>Phenuiviridae<\/jats:italic> nucleoprotein is the main structural and functional component of the viral cycle, protecting the viral RNA and mediating the essential replication\/transcription processes. The nucleoprotein (N) binds the RNA using its globular core and polymerizes through the N-terminus, which is presented as a highly flexible arm, as demonstrated in this article. The nucleoprotein exists in an `open' or a `closed' conformation. In the case of the closed conformation the flexible N-terminal arm folds over the RNA-binding cleft, preventing RNA adsorption. In the open conformation the arm is extended in such a way that both RNA adsorption and N polymerization are possible. In this article, single-crystal X-ray diffraction and small-angle X-ray scattering were used to study the N protein of Toscana virus complexed with a single-chain camelid antibody (VHH) and it is shown that in the presence of the antibody the nucleoprotein is unable to achieve a functional assembly to form a ribonucleoprotein complex.<\/jats:p>","DOI":"10.1107\/s2059798324000196","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T17:03:33Z","timestamp":1706115813000},"page":"113-122","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Structural flexibility of Toscana virus nucleoprotein in the presence of a single-chain camelid antibody"],"prefix":"10.1107","volume":"80","author":[{"given":"Nicolas","family":"Papageorgiou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Baklouti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Lichi\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Desmyter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4924-1991","authenticated-orcid":true,"given":"Bruno","family":"Canard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Coutard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8351-4992","authenticated-orcid":true,"given":"Fran\u00e7ois","family":"Ferron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,1,24]]},"reference":[{"key":"jc5062_bb2","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1107\/S2059798317008774","volume":"73","author":"Baklouti","year":"2017","journal-title":"Acta Cryst. D"},{"key":"jc5062_bb3","first-page":"183","volume":"3","author":"Bishop","year":"1986","journal-title":"Microbiol. Sci."},{"key":"jc5062_bb4","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.3201\/eid1111.050869","volume":"11","author":"Charrel","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"jc5062_bb5","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1140\/epjp\/i2017-11403-3","volume":"132","author":"Coati","year":"2017","journal-title":"Eur. Phys. J. Plus"},{"key":"jc5062_bb6","first-page":"22","volume":"4","author":"Cusi","year":"2010","journal-title":"Open Virol. J."},{"key":"jc5062_bb7","doi-asserted-by":"crossref","first-page":"E1371","DOI":"10.1073\/pnas.1301336110","volume":"110","author":"Desmyter","year":"2013","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"jc5062_bb8","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"jc5062_bb9","doi-asserted-by":"crossref","first-page":"e1002030","DOI":"10.1371\/journal.ppat.1002030","volume":"7","author":"Ferron","year":"2011","journal-title":"PLoS Pathog."},{"key":"jc5062_bb10","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.virusres.2017.01.018","volume":"234","author":"Ferron","year":"2017","journal-title":"Virus Res."},{"key":"jc5062_bb11","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1107\/S0021889809043076","volume":"43","author":"Fischer","year":"2010","journal-title":"J. Appl. Cryst."},{"key":"jc5062_bb13","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/0042-6822(91)90087-R","volume":"180","author":"Giorgi","year":"1991","journal-title":"Virology"},{"key":"jc5062_bb14","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1128\/jvi.21.3.1085-1093.1977","volume":"21","author":"Hewlett","year":"1977","journal-title":"J. Virol."},{"key":"jc5062_bb15","doi-asserted-by":"crossref","first-page":"6829","DOI":"10.1128\/JVI.00672-13","volume":"87","author":"Jiao","year":"2013","journal-title":"J. Virol."},{"key":"jc5062_bb66","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1107\/S0907444909047374","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"jc5062_bb16","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1107\/S0021889803012779","volume":"36","author":"Konarev","year":"2003","journal-title":"J. Appl. Cryst."},{"key":"jc5062_bb17","doi-asserted-by":"crossref","unstructured":"Kozlov, M. (2021). Nature, https:\/\/doi.org\/10.1038\/d41586-021-03829-0.","DOI":"10.1038\/d41586-021-03829-0"},{"key":"jc5062_bb18","doi-asserted-by":"crossref","first-page":"e936","DOI":"10.1371\/journal.pntd.0000936","volume":"5","author":"Lantez","year":"2011","journal-title":"PLoS Negl. Trop. Dis."},{"key":"jc5062_bb19","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1021\/ci200227u","volume":"51","author":"Laskowski","year":"2011","journal-title":"J. Chem. Inf. Model."},{"key":"jc5062_bb20","doi-asserted-by":"crossref","unstructured":"Laude, H. & Masters, P. S. (1995). The Coronaviridae, edited by S. G. Siddell, pp. 141-163. Boston: Springer.","DOI":"10.1007\/978-1-4899-1531-3_7"},{"key":"jc5062_bb21","doi-asserted-by":"crossref","first-page":"3512","DOI":"10.1093\/emboj\/17.13.3512","volume":"17","author":"Lauwereys","year":"1998","journal-title":"EMBO J."},{"key":"jc5062_bb22","doi-asserted-by":"crossref","first-page":"11974","DOI":"10.1128\/JVI.79.18.11974-11980.2005","volume":"79","author":"Le May","year":"2005","journal-title":"J. Virol."},{"key":"jc5062_bb1","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Cryst. D"},{"key":"jc5062_bb12","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1107\/S1600576720013412","volume":"54","author":"Manalastas-Cantos","year":"2021","journal-title":"J. Appl. Cryst."},{"key":"jc5062_bb23","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Cryst."},{"key":"jc5062_bb24","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.1093\/nar\/gku229","volume":"42","author":"Olal","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"jc5062_bb25","doi-asserted-by":"crossref","first-page":"772","DOI":"10.3390\/v12070772","volume":"12","author":"Papageorgiou","year":"2020","journal-title":"Viruses"},{"key":"jc5062_bb26","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.tim.2015.07.005","volume":"23","author":"Pelegrin","year":"2015","journal-title":"Trends Microbiol."},{"key":"jc5062_bb70","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1529\/biophysj.105.064154","volume":"89","author":"Petoukhov","year":"2005","journal-title":"Biophys. J."},{"key":"jc5062_bb27","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.sbi.2007.06.009","volume":"17","author":"Petoukhov","year":"2007","journal-title":"Curr. Opin. Struct. Biol."},{"key":"jc5062_bb28","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"jc5062_bb29","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1007\/s42770-023-00982-8","volume":"54","author":"Qian","year":"2023","journal-title":"Braz. J. Microbiol."},{"key":"jc5062_bb31","doi-asserted-by":"crossref","first-page":"19208","DOI":"10.1073\/pnas.1213553109","volume":"109","author":"Raymond","year":"2012","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"jc5062_bb30","doi-asserted-by":"crossref","first-page":"11769","DOI":"10.1073\/pnas.1001760107","volume":"107","author":"Raymond","year":"2010","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"jc5062_bb32","doi-asserted-by":"crossref","first-page":"W320","DOI":"10.1093\/nar\/gku316","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"jc5062_bb33","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1126\/science.3285470","volume":"240","author":"Skerra","year":"1988","journal-title":"Science"},{"key":"jc5062_bb67","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1016\/S0006-3495(99)77443-6","volume":"76","author":"Svergun","year":"1999","journal-title":"Biophys. J."},{"key":"jc5062_bb34","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1126\/science.2047874","volume":"252","author":"Waldmann","year":"1991","journal-title":"Science"},{"key":"jc5062_bb35","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s13238-013-3901-4","volume":"4","author":"Zhou","year":"2013","journal-title":"Protein Cell"}],"container-title":["Acta Crystallographica Section D Structural Biology"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/d\/issues\/2024\/02\/00\/jc5062\/jc5062.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/d\/issues\/2024\/02\/00\/jc5062\/jc5062.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T11:12:57Z","timestamp":1738753977000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2059798324000196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,24]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["S2059798324000196"],"URL":"https:\/\/doi.org\/10.1107\/s2059798324000196","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2059798324000196\/jc5062sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb8rcq\/pdb","asserted-by":"subject"}]},"ISSN":["2059-7983"],"issn-type":[{"value":"2059-7983","type":"print"}],"subject":[],"published":{"date-parts":[[2024,1,24]]},"assertion":[{"value":"Acta Crystallographica Section D: Structural Biology","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?jc5062","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"6 October 2023","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"5 January 2024","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"24 January 2024","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?jc5062"}},{"value":"\u00a9 2024 Nicolas Papageorgiou et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}