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The enzymatic product of CdaA, cyclic di-AMP, is a secondary messenger that is essential for the viability of many bacteria. Its absence in humans makes CdaA a very promising and attractive target for the development of new antibiotics. Here, the structural results are presented of a crystallographic fragment screen against CdaA from\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    , a saprophytic Gram-positive bacterium and an opportunistic food-borne pathogen that can cause listeriosis in humans and animals. Two of the eight fragment molecules reported here were localized in the highly conserved ATP-binding site. These fragments could serve as potential starting points for the development of antibiotics against several CdaA-dependent bacterial species.\n                  <\/jats:p>","DOI":"10.1107\/s205979832400336x","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T11:55:04Z","timestamp":1713873304000},"page":"350-361","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["A small step towards an important goal: fragment screen of the c-di-AMP-synthesizing enzyme CdaA"],"prefix":"10.1107","volume":"80","author":[{"given":"Piotr","family":"Neumann","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana L.","family":"Heidemann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Wollenhaupt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Dickmanns","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Agthe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00g30e956","id-type":"ROR","asserted-by":"publisher"}],"department":["Institut f\u00fcr Nanostruktur- und Festk\u00f6rperphysik"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-7047","authenticated-orcid":true,"given":"Manfred S.","family":"Weiss","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1739-6086","authenticated-orcid":true,"given":"Ralf","family":"Ficner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"ag5049_bb1","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1107\/S2059798318006551","volume":"74","author":"Afonine","year":"2018","journal-title":"Acta Cryst. 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