{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:44Z","timestamp":1785421664818,"version":"3.56.0"},"reference-count":37,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32201025"],"award-info":[{"award-number":["32201025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471311"],"award-info":[{"award-number":["32471311"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2025,3,27]]},"published-print":{"date-parts":[[2025,5,1]]},"abstract":"<jats:p>\n                    The surface protein P113 serves as a membrane-anchored protein that tethers the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    RH5 complex, including its associated partners CyRPA and RIPR, to the parasite surface. This anchoring mechanism ensures the proper localization and stabilization of RH5, facilitating its critical interaction with the host erythrocyte receptor basigin during erythrocyte invasion. Here, the helical-rich domain of P113 (residues 311\u2013679) from a\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species was expressed, purified and crystallized to elucidate its structural and functional characteristics. The recombinant protein, with a molecular weight of approximately 44\u2005kDa, was confirmed to be monomeric in solution. Crystallization in 0.5\u2005m\n                    <jats:italic>M<\/jats:italic>\n                    MES pH 6.0, 22% PEG 3350 yielded high-quality crystals, enabling the determination of the structure of the apo form at 1.7\u2005\u00c5 resolution. The structure revealed a predominant \u03b1-helical composition, with two distinct left-handed orthogonal four-helix bundles formed by helices \u03b11\u2013\u03b14 and \u03b16\u2013\u03b19 connected by a disordered region. Sequence analysis demonstrated high conservation of P113 across all human-infecting\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species, including\n                    <jats:italic>P. vivax<\/jats:italic>\n                    ,\n                    <jats:italic>P. malariae<\/jats:italic>\n                    ,\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    and\n                    <jats:italic>P. ovale<\/jats:italic>\n                    , as well as in\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species infecting primates and rodents. Protein\u2013protein interaction analysis using the\n                    <jats:italic>STRING<\/jats:italic>\n                    tool identified P113 as a hub protein that interacts with ten proteins, including small nuclear ribonucleoprotein, DNA polymerase delta small subunit and RIPR, which is part of the RH5\u2013CyRPA\u2013RIPR complex.\n                    <jats:italic>AlphaFold<\/jats:italic>\n                    predictions further elucidated the interaction patterns, revealing moderate to strong interaction scores (0.39\u20130.74) with key partners. Notably, the helical-rich domain of P113 was identified as the critical binding region for PF3D7_0308000, with key interaction sites mapped to residues Asp475, Arg381, Lys386, Asn390, Asp392 and Lys533. These findings provide critical insights into the structural and functional roles of P113 and its interaction network, advancing our understanding of its molecular mechanisms in\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    biology.\n                  <\/jats:p>","DOI":"10.1107\/s2059798325002748","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T09:53:36Z","timestamp":1743674016000},"page":"212-222","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["The crystal structure of an uncharacterized domain of P113 from\n                    <i>Plasmodium falciparum<\/i>"],"prefix":"10.1107","volume":"81","author":[{"given":"Zhudi","family":"Yuan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Urology, State Key Laboratory of Biotherapy, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, Laboratory of Diabetes and Metabolism Research, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianglei","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, Laboratory of Diabetes and Metabolism Research, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Yin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, Laboratory of Diabetes and Metabolism Research, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghui","family":"Fu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Urology, State Key Laboratory of Biotherapy, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7589-8877","authenticated-orcid":true,"given":"Shiqian","family":"Qi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, Laboratory of Diabetes and Metabolism Research, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Tang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, Laboratory of Diabetes and Metabolism Research, West China Hospital"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"wan5004_bb1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"wan5004_bb2","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"wan5004_bb3","first-page":"164","volume":"119","author":"Anders","year":"1986","journal-title":"Ciba Found. 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World Malaria Report. Geneva: World Health Organization. https:\/\/www.who.int\/teams\/global-malaria-programme\/reports\/world-malaria-report-2024."},{"key":"wan5004_bb37","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nature13715","volume":"515","author":"Wright","year":"2014","journal-title":"Nature"}],"container-title":["Acta Crystallographica Section D Structural Biology"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/d\/issues\/2025\/05\/00\/wan5004\/wan5004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/d\/issues\/2025\/05\/00\/wan5004\/wan5004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T07:15:32Z","timestamp":1746515732000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2059798325002748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["S2059798325002748"],"URL":"https:\/\/doi.org\/10.1107\/s2059798325002748","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.2210\/pdb9lpt\/pdb","asserted-by":"subject"}]},"ISSN":["2059-7983"],"issn-type":[{"value":"2059-7983","type":"print"}],"subject":[],"published":{"date-parts":[[2025,4,7]]},"assertion":[{"value":"Acta Crystallographica Section D: Structural Biology","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?wan5004","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"25 February 2025","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"27 March 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"7 April 2025","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?wan5004"}},{"value":"\u00a9 Zhudi Yuan\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}