{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:17:48Z","timestamp":1785403068304,"version":"3.56.0"},"reference-count":35,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2021YFA0911000"],"award-info":[{"award-number":["2021YFA0911000"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32101230"],"award-info":[{"award-number":["32101230"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2025,10,17]]},"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>\n                    Methanol, a sustainable and cost-effective C1 compound, has been considered as a promising substrate for the biosynthesis of fuels and value-added chemicals. Synthetic methylotrophs have been developed by integrating natural methanol-assimilation pathways into non-native microbial hosts, with NAD\n                    <jats:sup>+<\/jats:sup>\n                    -dependent methanol dehydrogenases (MDHs) serving as attractive candidates for methanol oxidation. NAD\n                    <jats:sup>+<\/jats:sup>\n                    -dependent MDH1 from the methylotrophic bacterium\n                    <jats:italic>Bacillus methanolicus<\/jats:italic>\n                    MGA3 (\n                    <jats:italic>Bm<\/jats:italic>\n                    MDH1) is one of the extensively studied MDHs. Although structural models of\n                    <jats:italic>Bm<\/jats:italic>\n                    MDH1 had been proposed, its crystal structure had not been experimentally determined. In this study, the crystal structure of\n                    <jats:italic>Bm<\/jats:italic>\n                    MDH1 is reported at 3.0\u2005\u00c5 resolution.\n                    <jats:italic>Bm<\/jats:italic>\n                    MDH1 forms a decamer made up of five dimers, stabilized by ionic and hydrogen-bonding interactions. Each monomer exhibits a conserved fold which is typical of the type III alcohol dehydrogenase family, comprising an N-terminal \u03b1\/\u03b2 dinucleotide-binding domain and a C-terminal all-\u03b1 helical domain. Similar to other enzymes in this family, it has an NAD\n                    <jats:sup>+<\/jats:sup>\n                    -binding site formed by a Rossmann fold. As a metalloenzyme,\n                    <jats:italic>Bm<\/jats:italic>\n                    MDH1 features a metal ion in its active site, coordinated by three histidine residues (His197, His262 and His276) and one aspartate residue (Asp193). Enzyme-activity assays identified Mn\n                    <jats:sup>2+<\/jats:sup>\n                    as the most effective metal ion for supporting\n                    <jats:italic>in vitro<\/jats:italic>\n                    enzymatic activity. These findings provide essential structural insights for the rational engineering of methanol-utilizing biocatalysts, thereby advancing sustainable microbial biomanufacturing.\n                  <\/jats:p>","DOI":"10.1107\/s205979832500909x","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:33:04Z","timestamp":1760963584000},"page":"646-655","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structural studies of NAD\n                    <sup>+<\/sup>\n                    -dependent methanol dehydrogenase 1 from\n                    <i>Bacillus methanolicus<\/i>\n                    MGA3"],"prefix":"10.1107","volume":"81","author":[{"given":"Bao-Di","family":"Ma","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Zhangjiang Institute for Advanced Study"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu-Dong","family":"Kong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0220qvk04","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, and Zhangjiang Institute for Advanced Study"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"cb5155_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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