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Therefore, the enzyme is a potential target for the development of antischistosomal inhibitors.\n                    <jats:italic>Sm<\/jats:italic>\n                    CD1 has significant sequence identity to cathepsin D-like proteases found in other schistosome species and homology to mammalian aspartic proteases. Here, we report the first crystal structures of a helminth cathepsin D,\n                    <jats:italic>Sm<\/jats:italic>\n                    CD1, and have identified a single-domain antibody (nanobody) that specifically binds to\n                    <jats:italic>Sm<\/jats:italic>\n                    CD1 with nanomolar affinity but does not recognize human cathepsin D. We have mapped the epitope of the nanobody by determining the crystal structure of the enzyme\u2013nanobody complex, revealing the conformation of\n                    <jats:italic>Sm<\/jats:italic>\n                    CD1 in the propeptide-bound state.\n                  <\/jats:p>","DOI":"10.1107\/s2059798326000422","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T12:15:14Z","timestamp":1768997714000},"page":"140-150","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Crystal structure of\n                    <i>Schistosoma mansoni<\/i>\n                    cathepsin D1 in complex with a nanobody reveals the conformation of the propeptide-bound state"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4877-4745","authenticated-orcid":true,"given":"Kelly L.","family":"Parker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7891-7626","authenticated-orcid":true,"given":"John D.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara F.","family":"Gomes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04jhswv08","id-type":"ROR","asserted-by":"publisher"}],"department":["LaBECFar \u2013 Laboratory of Experimental and Computational Biochemistry of Drugs"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.-A.","family":"Eyssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Furnham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floriano","family":"Paes Silva-Jr","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04jhswv08","id-type":"ROR","asserted-by":"publisher"}],"department":["LaBECFar \u2013 Laboratory of Experimental and Computational Biochemistry of Drugs"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3705-2993","authenticated-orcid":true,"given":"Raymond J.","family":"Owens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"ag5062_bb1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/s42003-023-04927-7","volume":"6","author":"Abanades","year":"2023","journal-title":"Commun. 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Parker\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}