{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:41:11Z","timestamp":1793313671316,"version":"4.5.0"},"reference-count":25,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJER2202"],"award-info":[{"award-number":["JPMJER2202"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21H05247"],"award-info":[{"award-number":["JP21H05247"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K18106"],"award-info":[{"award-number":["JP24K18106"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24KF0141"],"award-info":[{"award-number":["JP24KF0141"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23ama121002"],"award-info":[{"award-number":["JP23ama121002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2026,3,18]]},"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>\n                    Endogenous\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    proteins can co-purify with recombinant targets and dominate cryo-EM datasets, yet often escape detection during standard biochemical quality control. In parallel to the recent report by Caliseki and coworkers [Caliseki\n                    <jats:italic>et al.<\/jats:italic>\n                    (2025),\n                    <jats:italic>Acta Cryst.<\/jats:italic>\n                    D\n                    <jats:bold>81<\/jats:bold>\n                    , 545\u2013557], we independently identified ArnA contamination while purifying a soluble, low-yield KIF17\u2013IFT70 complex, ultimately obtaining a 3.23\u2005\u00c5 resolution cryo-EM structure of ArnA rather than the intended target. Our results reinforce that ArnA enrichment reflects general features of His-tag affinity purification and can become particularly problematic in cryo-EM workflows when the intended target is low in yield or conformationally heterogeneous. By comparing biochemical behavior and cryo-EM outcomes, we outline why ArnA may evade SDS\u2013PAGE and size-exclusion chromatography, and be underappreciated in routine mass spectrometry-based quality control, yet becomes structurally dominant in cryo-EM. These findings broaden the scope of the original study and highlight the need for early cryo-EM screening and improved contaminant awareness in structural biology.\n                  <\/jats:p>","DOI":"10.1107\/s2059798326002846","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T10:38:20Z","timestamp":1775817500000},"page":"404-410","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Cryo-EM reveals ArnA contamination during purification of a ciliary protein complex"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7997-872X","authenticated-orcid":true,"given":"Xuguang","family":"Jiang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Cell Biology and Anatomy, Graduate School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7656-8194","authenticated-orcid":true,"given":"Masahide","family":"Kikkawa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Cell Biology and Anatomy, Graduate School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"key":"jb5071_bb100","doi-asserted-by":"crossref","first-page":"e0180163","DOI":"10.1371\/journal.pone.0180163","volume":"12","author":"Abeyrathne","year":"2017","journal-title":"PLoS One"},{"key":"jb5071_bb1","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1002\/prot.24364","volume":"81","author":"Andersen","year":"2013","journal-title":"Proteins"},{"key":"jb5071_bb2","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.bbagen.2006.03.027","volume":"1760","author":"Bolanos-Garcia","year":"2006","journal-title":"Biochim. 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