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Microtubules can also adopt variable lattice architectures both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and in cellular contexts. Pseudo-helical averaging processing strategies have been developed to generate cryo-EM reconstructions of microtubules with and without decorating protein-binding partners, but these pipelines can be difficult to implement for the average user, especially for undecorated filaments. Here, we describe\n                    <jats:italic>MiCSPARC<\/jats:italic>\n                    , a cryo-EM processing pipeline developed around\n                    <jats:italic>CryoSPARC<\/jats:italic>\n                    [Punjani\n                    <jats:italic>et al.<\/jats:italic>\n                    (2017),\n                    <jats:italic>Nat. Methods<\/jats:italic>\n                    ,\n                    <jats:bold>14<\/jats:bold>\n                    , 290\u2013296], which leverages automated particle picking and fast 3D refinement times in\n                    <jats:italic>CryoSPARC<\/jats:italic>\n                    to determine the structures of both decorated and undecorated microtubules. We generate reconstructions of undecorated GDP microtubules, as well as kinesin-1 motor domain-decorated GMPCPP filaments, at resolutions of up to 2.8\u2005\u00c5, demonstrating the robustness of the pipeline. Based on its convenient implementation and its ability to routinely generate high-resolution, seam-corrected microtubule reconstructions,\n                    <jats:italic>MiCSPARC<\/jats:italic>\n                    should provide a valuable tool for understanding microtubule dynamics, microtubule-associated proteins and microtubule-targeting agents.\n                  <\/jats:p>","DOI":"10.1107\/s2059798326003062","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T10:07:58Z","timestamp":1776852478000},"page":"411-420","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["A cryo-EM processing pipeline for microtubules using\n                    <i>CryoSPARC<\/i>"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3708-5288","authenticated-orcid":true,"given":"Daniel","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a28rw58","id-type":"ROR","asserted-by":"publisher"}],"department":["Institute of Molecular Biology and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1193-4602","authenticated-orcid":true,"given":"Hugo","family":"Mu\u00f1oz-Hern\u00e1ndez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a28rw58","id-type":"ROR","asserted-by":"publisher"}],"department":["Institute of Molecular Biology and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1958-0458","authenticated-orcid":true,"given":"Pavel","family":"Filipcik","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03eh3y714","id-type":"ROR","asserted-by":"publisher"}],"department":["PSI Center for Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kushal","family":"Sejwal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03eh3y714","id-type":"ROR","asserted-by":"publisher"}],"department":["PSI Center for Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Xu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a28rw58","id-type":"ROR","asserted-by":"publisher"}],"department":["Institute of Molecular Biology and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Ryul","family":"Choi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03eh3y714","id-type":"ROR","asserted-by":"publisher"}],"department":["PSI Center for Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel O.","family":"Steinmetz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03eh3y714","id-type":"ROR","asserted-by":"publisher"}],"department":["PSI Center for Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3067-7314","authenticated-orcid":true,"given":"Michal","family":"Wieczorek","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a28rw58","id-type":"ROR","asserted-by":"publisher"}],"department":["Institute of Molecular Biology and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,4,27]]},"reference":[{"key":"zi5010_bb1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nrm4084","volume":"16","author":"Akhmanova","year":"2015","journal-title":"Nat. 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