{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:14:29Z","timestamp":1788340469057,"version":"build-2803163510"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["UKR11958"],"award-info":[{"award-number":["UKR11958"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["198466\/Z\/15\/Z"],"award-info":[{"award-number":["198466\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000777","name":"University of Leeds","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000777","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2026,7,10]]},"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>There has been a rapid expansion in new approaches to sample preparation for single-particle cryoEM. One drive has been around speeding up the process both for the application of time-resolved studies and in outrunning some of the negative effects which may come from the sample interacting with the air\u2013water interface, such as degradation and preferred orientation. Here, we set out to significantly improve the speed of grid preparation by removing the plunging stage and directly spraying onto pre-cooled grids which are situated in the cryogen, allowing us to produce grids where two samples are mixed and frozen on the grid in less than 1\u2005ms. We have previously shown a preliminary proof of principle with this new approach and here we provide a detailed description of the approach taken and the methodology behind it. This approach allows us to move beyond the millisecond timescale to open up more systems to time-resolved EM and potentially improve samples.<\/jats:p>","DOI":"10.1107\/s2059798326007126","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T13:33:43Z","timestamp":1786023223000},"page":"1056-1061","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Direct deposition on pre-cooled grids: a new approach to sub-millisecond cryoEM grid preparation"],"prefix":"10.1107","volume":"82","author":[{"given":"David P.","family":"Klebl","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biomedical Sciences, Faculty of Biomedical Sciences and Astbury Centre for Structural and Molecular Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Kay","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Mechanical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6833-5523","authenticated-orcid":true,"given":"Sean","family":"McMillan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biomedical Sciences, Faculty of Biomedical Sciences and Astbury Centre for Structural and Molecular Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9029-1865","authenticated-orcid":true,"given":"Frank","family":"Sobott","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural and Molecular Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1041-8390","authenticated-orcid":true,"given":"Nikil","family":"Kapur","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Mechanical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6869-4414","authenticated-orcid":true,"given":"Stephen P.","family":"Muench","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Biomedical Sciences, Faculty of Biomedical Sciences and Astbury Centre for Structural and Molecular Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"eh5026_bb1","doi-asserted-by":"crossref","first-page":"7705","DOI":"10.1038\/s41467-024-52091-1","volume":"15","author":"Abe","year":"2024","journal-title":"Nat. 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Klebl\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}