{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:49:44Z","timestamp":1793274584583,"version":"4.5.0"},"reference-count":57,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2026,9,21]]},"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:p>\n                    Catalases are ubiquitous enzymes that protect cells from oxidative stress through the disproportionation of hydrogen peroxide. Although oxidase side activities have been reported for a limited number of catalases, their structural basis and physiological relevance remain poorly understood. This study investigates the catalytic promiscuity of\n                    <jats:italic>Cl<\/jats:italic>\n                    CPO, an extracellular fungal catalase from the marine fungus\n                    <jats:italic>Cladosporium<\/jats:italic>\n                    sp., previously identified as a potential degrader of persistent organic pollutants. Recombinant\n                    <jats:italic>Cl<\/jats:italic>\n                    CPO was biochemically characterized and shown to catalyse the oxidation of\n                    <jats:italic>ortho<\/jats:italic>\n                    -diphenols to\n                    <jats:italic>ortho<\/jats:italic>\n                    -quinones, bearing hydroxy, ethylamine and single and double chlorine substitutions on the benzene ring, in the absence of hydrogen peroxide, while retaining robust catalase activity. On the contrary, it did not exhibit any cresolase activity in either unsubstituted or chlorinated monophenols, nor phenol oxidative activity on hydroxy-biphenyls. Crystal structures of apo\n                    <jats:italic>Cl<\/jats:italic>\n                    CPO and a catechol-bound complex were determined at high resolution, identifying a catechol-binding site located at the junction of the main and secondary access channels to the haem cofactor. Docking simulations suggested an additional candidate ligand-binding region capable of accommodating bulkier compounds, whereas\n                    <jats:italic>in silico<\/jats:italic>\n                    electron\/hole-transfer pathway analysis identified recurrent predicted pathway elements connecting the different ligand-binding regions to the haem centre. Structural comparison with previously characterized bifunctional catalases highlights distinct substrate-recognition features and suggests an alternative mechanistic basis for phenol oxidase activity in\n                    <jats:italic>Cl<\/jats:italic>\n                    CPO. Together, these findings provide structural insight into the catalytic promiscuity of\n                    <jats:italic>Cl<\/jats:italic>\n                    CPO, support a working mechanistic framework for phenol oxidase activity in fungal catalases and expand our understanding of the functional diversity of haem-dependent oxidoreductases.\n                  <\/jats:p>","DOI":"10.1107\/s2059798326009848","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:51:04Z","timestamp":1791197464000},"page":"1224-1239","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Beyond monofunctionality: structural perspectives on phenol oxidase side activity in a catalase"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-7373","authenticated-orcid":true,"given":"Christina","family":"Ferousi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cx6bg69","id-type":"ROR","asserted-by":"publisher"}],"department":["Industrial Biotechnology and Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5479-7858","authenticated-orcid":true,"given":"Christos","family":"Kosinas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7250-1691","authenticated-orcid":true,"given":"Odyssefs Ioannis","family":"Pantelakis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00d7xrm67","id-type":"ROR","asserted-by":"publisher"}],"department":["Institute of Molecular Biotechnology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8022-9272","authenticated-orcid":true,"given":"Efstratios","family":"Nikolaivits","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cx6bg69","id-type":"ROR","asserted-by":"publisher"}],"department":["Industrial Biotechnology and Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-5904","authenticated-orcid":true,"given":"Evangelos","family":"Topakas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cx6bg69","id-type":"ROR","asserted-by":"publisher"}],"department":["Industrial Biotechnology and Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5663-1421","authenticated-orcid":true,"given":"Maria","family":"Dimarogona","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/017wvtq80","id-type":"ROR","asserted-by":"publisher"}],"department":["Laboratory of Structural Biology and Biotechnology, Department of Chemical Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"nz5023_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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