{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:49:37Z","timestamp":1793274577847,"version":"4.5.0"},"reference-count":36,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD030275"],"award-info":[{"award-number":["S10OD030275"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM141119"],"award-info":[{"award-number":["R01GM141119"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT-190195"],"award-info":[{"award-number":["PJT-190195"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2022-04774"],"award-info":[{"award-number":["RGPIN-2022-04774"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"accepted":{"date-parts":[[2026,9,29]]},"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:p>\n                    Microtubules are cytoskeletal filaments that are involved in intracellular transport, cell division and motility. Despite their biological importance, determining their high-resolution structures via cryo-electron microscopy remains a significant technical challenge due to their polymorphism and pseudo-helical assembly. Current processing workflows are complex, often requiring the integration of multiple software packages and custom scripts, which creates a steep learning curve for many research groups. To address these limitations, we introduce\n                    <jats:italic>CsMT<\/jats:italic>\n                    , a streamlined workflow implemented entirely within the\n                    <jats:italic>CryoSPARC<\/jats:italic>\n                    environment and using synthetic references.\n                    <jats:italic>CsMT<\/jats:italic>\n                    simplifies microtubule reconstruction by utilizing a novel protofilament-pair classification approach, which effectively handles the inherent pseudo-symmetry and structural heterogeneity of microtubules with minimal manual intervention. Our workflow is versatile, and is capable of processing both undecorated and decorated microtubules while accurately determining seams and performing high-resolution refinement. We demonstrate the efficacy of this workflow by achieving 2.3 and 2.7\u2005\u00c5 resolution reconstructions of homotypic and heterotypic maps of undecorated microtubules, matching the best-resolved microtubule structures in the field. By unifying the pipeline into a single and portable workflow,\n                    <jats:italic>CsMT<\/jats:italic>\n                    enhances reproducibility and accessibility, empowering more laboratories to explore the structural biology of microtubules and associated proteins, yielding new insights into their function.\n                  <\/jats:p>","DOI":"10.1107\/s2059798326010144","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:33:01Z","timestamp":1791459181000},"page":"1212-1223","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["<i>CsMT<\/i>\n                    : a robust and streamlined\n                    <i>CryoSPARC<\/i>\n                    workflow for cryo-EM reconstruction of microtubules"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0967-4497","authenticated-orcid":true,"given":"Tina","family":"Alagha","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Anatomy and Cell Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3518-0510","authenticated-orcid":true,"given":"Asuva","family":"Arin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Anatomy and Cell Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3861-9242","authenticated-orcid":true,"given":"Nicholas","family":"Vangos","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"publisher"}],"department":["Life Sciences Institute"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8460-9125","authenticated-orcid":true,"given":"Helena","family":"Goodey-Parfitt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Anatomy and Cell Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6804-4646","authenticated-orcid":true,"given":"Hai Nguyen","family":"Ngo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qaa6j11","id-type":"ROR","asserted-by":"publisher"}],"department":["Faculty of Fundamental Sciences, Hue University of Medicine and Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhat Nam","family":"Dau","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qaa6j11","id-type":"ROR","asserted-by":"publisher"}],"department":["Faculty of Fundamental Sciences, Hue University of Medicine and Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8257-6032","authenticated-orcid":true,"given":"Minh Hoa","family":"Nguyen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qaa6j11","id-type":"ROR","asserted-by":"publisher"}],"department":["Faculty of Fundamental Sciences, Hue University of Medicine and Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1477-1722","authenticated-orcid":true,"given":"Thibault","family":"Legal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Anatomy and Cell Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2067-4999","authenticated-orcid":true,"given":"Michael A.","family":"Cianfrocco","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jmfr291","id-type":"ROR","asserted-by":"publisher"}],"department":["Life Sciences Institute"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2814-9889","authenticated-orcid":true,"given":"Khanh Huy","family":"Bui","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Anatomy and Cell Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"sor5009_bb1","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1107\/S2059798318009324","volume":"74","author":"Afonine","year":"2018","journal-title":"Acta Cryst. 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