{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:06:33Z","timestamp":1762506393431,"version":"3.33.0"},"reference-count":43,"publisher":"International Union of Crystallography (IUCr)","issue":"8","license":[{"start":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:00:00Z","timestamp":1406246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"},{"start":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:00:00Z","timestamp":1406246400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Biol Crystallogr","Acta Crystallogr D","Acta Crystallogr D Biol Cryst","Acta Cryst D","Acta Cryst D Biol Crystallogr","Acta Cryst D Biol Cryst","Acta Cryst Sect D","Acta Cryst Sect D Biol Cryst","Acta Crystallogr Sect D","Acta Crystallogr Sect D Biol Crystallogr"],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>Uracil-DNA<jats:italic>N<\/jats:italic>-glycosylase from Atlantic cod (cUNG) shows cold-adapted features such as high catalytic efficiency, a low temperature optimum for activity and reduced thermal stability compared with its mesophilic homologue human UNG (hUNG). In order to understand the role of the enzyme\u2013substrate interaction related to the cold-adapted properties, the structure of cUNG in complex with a bacteriophage encoded natural UNG inhibitor (Ugi) has been determined. The interaction has also been analyzed by isothermal titration calorimetry (ITC). The crystal structure of cUNG\u2013Ugi was determined to a resolution of 1.9\u2005\u00c5 with eight complexes in the asymmetric unit related through noncrystallographic symmetry. A comparison of the cUNG\u2013Ugi complex with previously determined structures of UNG\u2013Ugi shows that they are very similar, and confirmed the nucleotide-mimicking properties of Ugi. Biophysically, the interaction between cUNG and Ugi is very strong and shows a binding constant (<jats:italic>K<\/jats:italic><jats:sub>b<\/jats:sub>) which is one order of magnitude larger than that for hUNG\u2013Ugi. The binding of both cUNG and hUNG to Ugi was shown to be favoured by both enthalpic and entropic forces; however, the binding of cUNG to Ugi is mainly dominated by enthalpy, while the entropic term is dominant for hUNG. The observed differences in the binding properties may be explained by an overall greater positive electrostatic surface potential in the protein\u2013Ugi interface of cUNG and the slightly more hydrophobic surface of hUNG.<\/jats:p>","DOI":"10.1107\/s1399004714011699","type":"journal-article","created":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T16:37:27Z","timestamp":1406306247000},"page":"2093-2100","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":7,"title":["Structural and biophysical analysis of interactions between cod and human uracil-DNA<i>N<\/i>-glycosylase (UNG) and UNG inhibitor (Ugi)"],"prefix":"10.1107","volume":"70","author":[{"given":"Netsanet Gizaw","family":"Assefa","sequence":"first","affiliation":[]},{"given":"Laila","family":"Niiranen","sequence":"additional","affiliation":[]},{"given":"Kenneth A.","family":"Johnson","sequence":"additional","affiliation":[]},{"given":"Hanna-Kirsti Schr\u00f8der","family":"Leiros","sequence":"additional","affiliation":[]},{"given":"Arne Oskar","family":"Smal\u00e5s","sequence":"additional","affiliation":[]},{"given":"Nils Peder","family":"Willassen","sequence":"additional","affiliation":[]},{"given":"Elin","family":"Moe","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2014,7,25]]},"reference":[{"key":"gm5031_bb1","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1099\/mic.0.26228-0","volume":"149","author":"Acharya","year":"2003","journal-title":"Microbiology"},{"key":"gm5031_bb2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/S0022-2836(02)00654-X","volume":"321","author":"Acharya","year":"2002","journal-title":"J. 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