{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T07:01:07Z","timestamp":1756191667837,"version":"3.33.0"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T00:00:00Z","timestamp":1397520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T00:00:00Z","timestamp":1397520000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,5,1]]},"abstract":"<jats:p>The truncated carbohydrate-active enzyme belonging to family 43 glycoside hydrolase from<jats:italic>Clostridium thermocellum<\/jats:italic>(<jats:italic>Ct<\/jats:italic>GH43) is an \u03b1-L-arabinofuranosidase that in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans. The recombinant enzyme<jats:italic>Ct<\/jats:italic>GH43 from<jats:italic>C. thermocellum<\/jats:italic>was overexpressed in<jats:italic>Escherichia coli<\/jats:italic>and purified by immobilized metal-ion affinity chromatography. The recombinant<jats:italic>Ct<\/jats:italic>GH43 has a molecular mass of 35.86\u2005kDa. Preliminary structural characterization was carried out on<jats:italic>Ct<\/jats:italic>GH43 crystallized from different conditions, which gave either cube-shaped or brick-shaped crystals. These diffracted to a resolution of 1.65\u2005\u00c5 for the cubic form and 1.1\u2005\u00c5 for the monoclinic form. Molecular replacement was used to solve the<jats:italic>Ct<\/jats:italic>GH43 structure.<\/jats:p>","DOI":"10.1107\/s2053230x14006402","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T15:51:08Z","timestamp":1397490668000},"page":"616-618","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":3,"title":["Crystallization and preliminary X-ray crystallographic analysis of a novel \u03b1-<scp>L<\/scp>-arabinofuranosidase (<i>Ct<\/i>GH43) from<i>Clostridium thermocellum<\/i>ATCC 27405"],"prefix":"10.1107","volume":"70","author":[{"given":"Arun","family":"Goyal","sequence":"first","affiliation":[]},{"given":"Shadab","family":"Ahmed","sequence":"additional","affiliation":[]},{"given":"Carlos M. G. 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