{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T05:11:44Z","timestamp":1736831504870,"version":"3.33.0"},"reference-count":15,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T00:00:00Z","timestamp":1399680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T00:00:00Z","timestamp":1399680000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>Plant cell-wall polysaccharides offer an abundant energy source utilized by many microorganisms, thus playing a central role in carbon recycling. Aerobic microorganisms secrete carbohydrate-active enzymes (CAZymes) that catabolize this composite structure, comprising cellulose, hemicellulose and lignin, into simple compounds such as glucose. Carbohydrate-binding modules (CBMs) enhance the efficacy of associated CAZYmes. They are organized into families based on primary-sequence homology. CBM family 46 contains more than 40 different members, but has yet to be fully characterized. Here, a recombinant derivative of the C-terminal family 46 CBM module (<jats:italic>Bh<\/jats:italic>CBM46) of<jats:italic>Bacillus halodurans<\/jats:italic>endo-\u03b2-1,4-glucanase B (CelB) was overexpressed in<jats:italic>Escherichia coli<\/jats:italic>and purified by immobilized metal-ion affinity chromatography. Preliminary structural characterization was carried out on<jats:italic>Bh<\/jats:italic>CBM46 crystallized in different conditions. The crystals of<jats:italic>Bh<\/jats:italic>CBM46 belonged to the tetragonal space group<jats:italic>I<\/jats:italic>4<jats:sub>1<\/jats:sub>22. Data were collected for the native form and a selenomethionine derivative to 2.46 and 2.3\u2005\u00c5 resolution, respectively. The<jats:italic>Bh<\/jats:italic>CBM46 structure was determined by a single-wavelength anomalous dispersion experiment using<jats:italic>AutoSol<\/jats:italic>from the<jats:italic>PHENIX<\/jats:italic>suite.<\/jats:p>","DOI":"10.1107\/s2053230x14008395","type":"journal-article","created":{"date-parts":[[2014,5,9]],"date-time":"2014-05-09T13:41:06Z","timestamp":1399642866000},"page":"754-757","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-\u03b2-1,4-glucanase B (CelB) from<i>Bacillus halodurans<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Immacolata","family":"Venditto","sequence":"first","affiliation":[]},{"given":"Helena","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds M. A.","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Kazuo","family":"Sakka","sequence":"additional","affiliation":[]},{"given":"Carlos M. G. A.","family":"Fontes","sequence":"additional","affiliation":[]},{"given":"Shabir","family":"Najmudin","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2014,5,10]]},"reference":[{"key":"bo5134_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. D"},{"key":"bo5134_bb2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","volume":"67","author":"Battye","year":"2011","journal-title":"Acta Cryst. D"},{"key":"bo5134_bb3","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1042\/BJ20040892","volume":"382","author":"Boraston","year":"2004","journal-title":"Biochem. 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Cryst."},{"key":"bo5134_bb10","doi-asserted-by":"crossref","first-page":"8815","DOI":"10.1074\/jbc.M510559200","volume":"281","author":"Najmudin","year":"2006","journal-title":"J. Biol. Chem."},{"key":"bo5134_bb11","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.jsb.2010.07.009","volume":"172","author":"Najmudin","year":"2010","journal-title":"J. Struct. Biol."},{"key":"bo5134_bb12","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.1093\/nar\/28.21.4317","volume":"28","author":"Takami","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"bo5134_bb13","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1107\/S0907444909012098","volume":"65","author":"Terwilliger","year":"2009","journal-title":"Acta Cryst. D"},{"key":"bo5134_bb14","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1107\/S090744490705024X","volume":"64","author":"Terwilliger","year":"2008","journal-title":"Acta Cryst. D"},{"key":"bo5134_bb15","doi-asserted-by":"crossref","first-page":"6851","DOI":"10.1128\/AEM.01209-06","volume":"72","author":"Wamalwa","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"bo5134_bb16","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/06\/00\/bo5134\/bo5134.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/06\/00\/bo5134\/bo5134.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T09:41:49Z","timestamp":1736761309000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X14008395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,10]]},"references-count":15,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6]]}},"alternative-id":["S2053230X14008395"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x14008395","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2014,5,10]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Journal","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"crystallization communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2011\/01\/00\/me0435\/index.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"5 February 2014","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"14 April 2014","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"10 May 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}