{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:56:45Z","timestamp":1758401805836,"version":"3.33.0"},"reference-count":22,"publisher":"International Union of Crystallography (IUCr)","issue":"8","license":[{"start":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T00:00:00Z","timestamp":1406073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T00:00:00Z","timestamp":1406073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>Cellulosomes are cell-bound multienzyme complexes secreted by anaerobic bacteria that play a crucial role in carbon turnover by degrading plant cell walls to simple sugars. Integration of cellulosomal components occurs<jats:italic>via<\/jats:italic>highly ordered protein\u2013protein interactions between cohesin modules located in a molecular scaffold and dockerin modules found in cellulosomal enzymes.<jats:italic>Acetivibrio cellulolyticus<\/jats:italic>possesses a complex cellulosome arrangement which is organized by a primary enzyme-binding scaffoldin (ScaA), two anchoring scaffoldins (ScaC and ScaD) and an unusual adaptor scaffoldin (ScaB). A dockerin from a family 5 glycoside hydrolase (GH5), which was engineered to inactivate one of the two putative cohesin-binding interfaces, complexed with one of the ScaA cohesins from<jats:italic>A. cellulolyticus<\/jats:italic>has been purified and crystallized, and data were processed to a resolution of 1.57\u2005\u00c5 in the orthorhombic space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>.<\/jats:p>","DOI":"10.1107\/s2053230x14013181","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T16:40:13Z","timestamp":1406047213000},"page":"1065-1067","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Overexpression, purification, crystallization and preliminary X-ray characterization of the fourth scaffoldin A cohesin from<i>Acetivibrio cellulolyticus<\/i>in complex with a dockerin from a family 5 glycoside hydrolase"],"prefix":"10.1107","volume":"70","author":[{"given":"Pedro","family":"Bule","sequence":"first","affiliation":[]},{"given":"Ana","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Kate","family":"Cameron","sequence":"additional","affiliation":[]},{"given":"Victor D.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"V\u00e2nia","family":"Cardoso","sequence":"additional","affiliation":[]},{"given":"Carlos M. 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