{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:09:07Z","timestamp":1761217747378,"version":"3.33.0"},"reference-count":23,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2014,11,14]],"date-time":"2014-11-14T00:00:00Z","timestamp":1415923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,11,14]],"date-time":"2014-11-14T00:00:00Z","timestamp":1415923200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>Cellulases catalyze the hydrolysis of cellulose, the major constituent of plant biomass and the most abundant organic polymer on earth. Cellulases are modular enzymes containing catalytic domains connected,<jats:italic>via<\/jats:italic>linker sequences, to noncatalytic carbohydrate-binding modules (CBMs). A putative modular endo-\u03b2-1,4-glucanase (<jats:italic>Bh<\/jats:italic>Cel5B) is encoded at locus BH0603 in the genome of<jats:italic>Bacillus halodurans<\/jats:italic>. It is composed of an N-terminal glycoside hydrolase family 5 catalytic module (GH5) followed by an immunoglobulin-like module and a C-terminal family 46 CBM (<jats:italic>Bh<\/jats:italic>CBM46). Here, the crystallization and preliminary X-ray diffraction analysis of the trimodular<jats:italic>Bh<\/jats:italic>Cel5B are reported. The crystals of<jats:italic>Bh<\/jats:italic>Cel5B belonged to the orthorhombic space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2 and data were processed to a resolution of 1.64\u2005\u00c5. A molecular-replacement solution has been found.<\/jats:p>","DOI":"10.1107\/s2053230x1402319x","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T16:19:03Z","timestamp":1415895543000},"page":"1628-1630","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-\u03b2-1,4-glucanase (Cel5B) from<i>Bacillus halodurans<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Immacolata","family":"Venditto","sequence":"first","affiliation":[]},{"given":"Helena","family":"Santos","sequence":"additional","affiliation":[]},{"given":"James","family":"Sandy","sequence":"additional","affiliation":[]},{"given":"Juan","family":"Sanchez-Weatherby","sequence":"additional","affiliation":[]},{"given":"Luis M. A.","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Kazuo","family":"Sakka","sequence":"additional","affiliation":[]},{"given":"Carlos M. G. A.","family":"Fontes","sequence":"additional","affiliation":[]},{"given":"Shabir","family":"Najmudin","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2014,11,14]]},"reference":[{"key":"pq5003_bb1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","volume":"67","author":"Battye","year":"2011","journal-title":"Acta Cryst. D"},{"key":"pq5003_bb2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev.micro.57.030502.091022","volume":"58","author":"Bayer","year":"2004","journal-title":"Annu. Rev. Microbiol."},{"key":"pq5003_bb22","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1107\/S0907444913001121","volume":"69","author":"Diederichs","year":"2013","journal-title":"Acta Cryst. 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Biochem."},{"key":"pq5003_bb12","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.pbi.2008.03.006","volume":"11","author":"Mohnen","year":"2008","journal-title":"Curr. Opin. Plant Biol."},{"key":"pq5003_bb13","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Cryst. D"},{"key":"pq5003_bb14","doi-asserted-by":"crossref","first-page":"8815","DOI":"10.1074\/jbc.M510559200","volume":"281","author":"Najmudin","year":"2006","journal-title":"J. Biol. Chem."},{"key":"pq5003_bb15","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.1093\/nar\/28.21.4317","volume":"28","author":"Takami","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"pq5003_bb16","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1107\/S2053230X14008395","volume":"70","author":"Venditto","year":"2014","journal-title":"Acta Cryst. 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