{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:53:45Z","timestamp":1778054025444,"version":"3.51.4"},"reference-count":18,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T00:00:00Z","timestamp":1417132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T00:00:00Z","timestamp":1417132800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>Endonuclease III is a bifunctional DNA glycosylase that removes a wide range of oxidized bases in DNA.<jats:italic>Deinococcus radiodurans<\/jats:italic>is an extreme radiation-resistant and desiccation-resistant bacterium and possesses three genes encoding endonuclease III enzymes in its genome: DR2438 (EndoIII-1), DR0289 (EndoIII-2) and DR0982 (EndoIII-3). Here, EndoIII-1 and an N-terminally truncated form of EndoIII-3 (EndoIII-3\u039476) have been expressed, purified and crystallized, and preliminary X-ray crystallographic analyses have been performed to 2.15 and 1.31\u2005\u00c5 resolution, respectively. The EndoIII-1 crystals belonged to the monoclinic space group<jats:italic>C<\/jats:italic>2, with unit-cell parameters<jats:italic>a<\/jats:italic>= 181.38,<jats:italic>b<\/jats:italic>= 38.56,<jats:italic>c<\/jats:italic>= 37.09\u2005\u00c5, \u03b2 = 89.34\u00b0 and one molecule per asymmetric unit. The EndoIII-3\u039476 crystals also belonged to the monoclinic space group<jats:italic>C<\/jats:italic>2, but with unit-cell parameters<jats:italic>a<\/jats:italic>= 91.47,<jats:italic>b<\/jats:italic>= 40.53,<jats:italic>c<\/jats:italic>= 72.47\u2005\u00c5, \u03b2 = 102.53\u00b0 and one molecule per asymmetric unit. The EndoIII-1 structure was determined by molecular replacement, while the truncated EndoIII-3\u039476 structure was determined by single-wavelength anomalous dispersion phasing. Refinement of the structures is in progress.<\/jats:p>","DOI":"10.1107\/s2053230x14024935","type":"journal-article","created":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T19:18:01Z","timestamp":1417202281000},"page":"1688-1692","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":6,"title":["Expression, purification and crystallization of two endonuclease III enzymes from<i>Deinococcus radiodurans<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Aili","family":"Sarre","sequence":"first","affiliation":[]},{"given":"Mats","family":"\u00d6kvist","sequence":"additional","affiliation":[]},{"given":"Tobias","family":"Klar","sequence":"additional","affiliation":[]},{"given":"Elin","family":"Moe","sequence":"additional","affiliation":[]},{"given":"Joanna","family":"Timmins","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2014,11,28]]},"reference":[{"key":"no5065_bb1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1146\/annurev.micro.51.1.203","volume":"51","author":"Battista","year":"1997","journal-title":"Annu. 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