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Excessive elastin proteolysis leads to a number of pathological diseases. Porcine pancreatic elastase (PPE) is often used for drug development as a model for human leukocyte elastase (HLE), with which it shares high sequence identity. Crystals of PPE were grown overnight using sodium sulfate and sodium acetate at acidic pH. Cross-linking the crystals with glutaraldehyde was needed to resist the soaking procedure with a diethyl <jats:italic>N<\/jats:italic>-(methyl)pyridinyl-substituted oxo-\u03b2-lactam inhibitor. Crystals of PPE bound to the inhibitor belonged to the orthorhombic space group <jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, with unit-cell parameters <jats:italic>a<\/jats:italic> = 51.0, <jats:italic>b<\/jats:italic> = 58.3, <jats:italic>c<\/jats:italic>\u00a0= 74.9\u2005\u00c5, and diffracted to 1.8\u2005\u00c5 resolution using an in-house X-ray source.<\/jats:p>","DOI":"10.1107\/s2053230x15017045","type":"journal-article","created":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T16:09:46Z","timestamp":1443024586000},"page":"1346-1351","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":8,"title":["Stabilization of porcine pancreatic elastase crystals by glutaraldehyde cross-linking"],"prefix":"10.1107","volume":"71","author":[{"given":"Stefan","family":"Hofbauer","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8307-2282","authenticated-orcid":true,"given":"Jos\u00e9 A.","family":"Brito","sequence":"additional","affiliation":[]},{"given":"Jalmira","family":"Mulchande","sequence":"additional","affiliation":[]},{"given":"Przemyslaw","family":"Nogly","sequence":"additional","affiliation":[]},{"given":"Miguel","family":"Pessanha","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Moreira","sequence":"additional","affiliation":[]},{"given":"Margarida","family":"Archer","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2015,9,23]]},"reference":[{"key":"wd5255_bb1","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1107\/S0907444909017855","volume":"65","author":"Andersen","year":"2009","journal-title":"Acta Cryst. 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