{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:22:13Z","timestamp":1740172933775,"version":"3.37.3"},"reference-count":22,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/92955\/2013","POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013","SFRH\/BPD\/108299\/2015"],"award-info":[{"award-number":["SFRH\/BD\/92955\/2013","POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013","SFRH\/BPD\/108299\/2015"]}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013","Norte-01-0145-FEDER-000012"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013","Norte-01-0145-FEDER-000012"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"COMPETE 2020 \u2013 Operational Programme for Competitiveness and Internationalization (POCI)","award":["POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007274","UID\/NEU\/04539\/2013"]}]},{"name":"Norte Portugal Regional Operational Programme (NORTE 2020)","award":["Norte-01-0145-FEDER-000012"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000012"]}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2017,9,1]]},"abstract":"<jats:p>Glucosylglycerate hydrolase is highly conserved among rapidly growing mycobacteria and has been found to be involved in recovery from nitrogen starvation by promoting the rapid mobilization of the glucosylglycerate that accumulates under these conditions. Here, the production, crystallization and structure determination of glucosylglycerate hydrolase from<jats:italic>Mycobacterium hassiacum<\/jats:italic>using two-wavelength anomalous diffraction of selenomethionine-substituted crystals are described. The monoclinic (space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>) crystals diffracted to \u223c2.0\u2005\u00c5 resolution at a synchrotron-radiation source and contained four molecules in the asymmetric unit, corresponding to a Matthews coefficient of 3.07\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup>and a solvent content of 59.9%. The quality of the experimental phases allowed the automated building of 1677 of the 1792 residues in the asymmetric unit.<\/jats:p>","DOI":"10.1107\/s2053230x17012419","type":"journal-article","created":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T15:27:01Z","timestamp":1504279621000},"page":"536-540","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Production, crystallization and structure determination of a mycobacterial glucosylglycerate hydrolase"],"prefix":"10.1107","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7176-0205","authenticated-orcid":true,"given":"Tatiana Barros","family":"Cereija","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1615-6099","authenticated-orcid":true,"given":"Susana","family":"Alarico","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8938-7560","authenticated-orcid":true,"given":"Nuno","family":"Empadinhas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0969-5438","authenticated-orcid":true,"given":"Pedro Jos\u00e9 Barbosa","family":"Pereira","sequence":"additional","affiliation":[]}],"member":"329","published-online":{"date-parts":[[2017,9,1]]},"reference":[{"key":"us5097_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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