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The role of Met405 in the active site of <jats:italic>Desulfovibrio vulgaris<\/jats:italic> formate dehydrogenase AB (<jats:italic>Dv<\/jats:italic>FdhAB) has remained elusive. However, its proximity to the metal site and the conformational change that it undergoes between the resting and active forms suggests a functional role. In this work, the M405S variant was engineered, which allowed the active-site geometry in the absence of methionine S<jats:sup>\u03b4<\/jats:sup> interactions with the metal site to be revealed and the role of Met405 in catalysis to be probed. This variant displayed reduced activity in both formate oxidation and CO<jats:sub>2<\/jats:sub> reduction, together with an increased sensitivity to oxygen inactivation.<\/jats:p>","DOI":"10.1107\/s2053230x24003911","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T09:55:17Z","timestamp":1714730117000},"page":"98-106","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structural and biochemical characterization of the M405S variant of <i>Desulfovibrio vulgaris<\/i> formate dehydrogenase"],"prefix":"10.1107","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0003-9637","authenticated-orcid":true,"given":"Guilherme","family":"Vilela-Alves","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology"]}]},{"given":"Rita","family":"Rebelo Manuel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier"]}]},{"given":"Neide","family":"Pedrosa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier"]}]},{"given":"In\u00eas A.","family":"Cardoso Pereira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3004-0543","authenticated-orcid":true,"given":"Maria Jo\u00e3o","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology"]}]},{"given":"Cristiano","family":"Mota","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology"]}]}],"member":"329","published-online":{"date-parts":[[2024,5,1]]},"reference":[{"key":"no5205_bb34","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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