{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:21:12Z","timestamp":1790079672063,"version":"4.0.1"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":2192,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cold\u2010shock proteins (CSPs) bind to single\u2010stranded nucleic acids, thereby acting as a \u201cRNA chaperone.\u201d To gain deeper insights into the rather unspecific nature of ssDNA\/RNA binding, we characterized the binding interface of CspB from <jats:italic>Bacillus subtilis<\/jats:italic> to a 25\u2010mer of ssDNA (Y\u2010Box25) using heteronuclear 2D NMR spectroscopy. Seventeen residues, including eight out of nine aromatic amino acids, are directly involved in the Y\u2010Box25 interaction and were identified by extreme line broadening of their cross\u2010peaks. Eight residues belong to the earlier proposed RNP binding motifs. A second set of seven backbone amides becomes evident by major chemical shift perturbations reporting remote conformational rearrangements upon binding. These residues are located in loop \u03b23\u2013\u03b24 and loop\u03b24\u2013\u03b25, and include Ile18. The individual contributions of the so\u2010identified residues were examined by fluorescence titration experiments of 15 CspB variants. Phenylalanine substitutions in\u2010 and outside the RNP motifs significantly reduce the binding affinity. Unrestricted possible backbone conformations of loop \u03b23\u2013\u03b24 also markedly contribute to binding. Stopped\u2010flow fluorescence kinetics revealed that the different binding affinities of CspB variants are determined by the dissociation rate, whereas the association rate remains unchanged. This might be of importance for the \u201cRNA chaperone\u201d activity of CspB.<\/jats:p>","DOI":"10.1110\/ps.0219703","type":"journal-article","created":{"date-parts":[[2003,8,27]],"date-time":"2003-08-27T22:41:40Z","timestamp":1062024100000},"page":"112-123","source":"Crossref","is-referenced-by-count":41,"title":["Single\u2010stranded DNA binding of the cold\u2010shock protein CspB from <i>Bacillus subtilis<\/i>: NMR mapping and mutational characterization"],"prefix":"10.1002","volume":"12","author":[{"given":"Markus","family":"Zeeb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Balbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00527a028"},{"key":"e_1_2_7_3_1","volume-title":"Relaxation kinetics","author":"Bernasconi C.F.","year":"1976"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1661"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.362897.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.6.1653"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.35501"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7322"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2902"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.381"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)78013-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi980269j"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0897-605"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90602-7"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.363898.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.24.7404"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80355-2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5121878.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01255-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9611164"},{"key":"e_1_2_7_21_1","first-page":"211","article-title":"Cold shock response and low temperature adaptation in psychrotrophic bacteria","volume":"1","author":"Hebraud M.","year":"1999","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.30.1.35"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)00252-3"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1651"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81720-1"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011296112710"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.18.5824-5828.1993"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.196"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00359.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.5.2092-2095.1987"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.18.5798-5802.1992"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101624200"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00052a088"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5334"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00009-4"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)08017-C"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00048-0"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.47.33601"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010474200"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1336"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90387-Y"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05726.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.6.1459"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5114"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi001174w"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(91)90036-S"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.5.1266"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01541.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)80031-9"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008605"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02192855"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409239309082571"},{"key":"e_1_2_7_53_1","volume-title":"Dynamic NMR spectroscopy","author":"Sandstr\u00f6m J.","year":"1982"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340140113"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/364164a0"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5119"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0895-663"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(19980301)30:4<401::AID-PROT7>3.0.CO;2-L"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3407"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00175254"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/364169a0"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(94)00096-4"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02431.x"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.6.1436"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.2.452"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90054-4"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)52050-X"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3693"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00683.x"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1110%2Fps.0219703","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1110\/ps.0219703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:02:10Z","timestamp":1697241730000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1110\/ps.0219703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":68,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1110\/ps.0219703"],"URL":"https:\/\/doi.org\/10.1110\/ps.0219703","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}