{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:23:38Z","timestamp":1789917818086,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":1371,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protein \u03b1\u2010Synuclein (aS) is a synaptic vesicle\u2010associated regulator of synaptic strength and dopamine homeostasis with a pathological role in Parkinson's disease. The normal function of aS depends on a membrane\u2010associated conformation that is adopted upon binding to negatively charged lipid surfaces. Previously we found that the membrane\u2010binding domain of aS is helical and suggested that it may exhibit an unusual structural periodicity. Here we present a study of the periodicity, topology, and dynamics of detergent micelle\u2010bound aS using paramagnetic spin labels embedded in the micelle or attached to the protein. We show that the helical region of aS completes three full turns every 11 residues, demonstrating the proposed 11\/3 periodicity. We also find that the membrane\u2010binding domain is partially buried in the micelle surface and bends toward the hydrophobic interior, but does not traverse the micelle. Deeper submersion of certain regions within the micelle, including the unique lysine\u2010free sixth 11\u2010residue repeat, is observed and may be functionally important. There are no long\u2010range tertiary contacts within this domain, indicating a highly extended configuration. The backbone dynamics of the micelle\u2010bound region are relatively uniform with a slight decrease in flexibility observed toward the C\u2010terminal end. These results clarify the topological features of aS bound to membrane\u2010mimicking detergent micelles, with implications for aS function and pathology.<\/jats:p>","DOI":"10.1110\/ps.041255905","type":"journal-article","created":{"date-parts":[[2005,3,2]],"date-time":"2005-03-02T03:48:38Z","timestamp":1109735318000},"page":"862-872","source":"Crossref","is-referenced-by-count":134,"title":["Helix periodicity, topology, and dynamics of membrane\u2010associated \u03b1\u2010Synuclein"],"prefix":"10.1002","volume":"14","author":[{"suffix":"Jr.","given":"Robert","family":"Bussell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trudy Fiona","family":"Ramlall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Eliezer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80886-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110414200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048448q"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/306281a0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106777200"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00520-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi036135"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-20-08797.2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M213128200"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja017875d"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108414200"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi991447r"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9443"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4538"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600211"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008919200"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.0954"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00514-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90302-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400553101"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi972776r"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.41.26292"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004345200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.2004.04250.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0022-2275(20)41549-4","article-title":"Computer programs to identify and classify amphipathic \u03b1 helical domains","volume":"33","author":"Jones M.K.","year":"1992","journal-title":"J. Lipid Res."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb10950.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fsb2fj000334com"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107045200"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3450401"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205518200"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.12.8812"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-09-03214.2000"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090439"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.02.014"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-08-03090.2002"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105022200"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi035048e"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(03)00036-8"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)41536-6"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.45.31755"},{"key":"e_1_2_7_42_1","first-page":"330","volume-title":"Current topics in membranes","author":"Segrest J.P.","year":"2002"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.9.4897"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/42166"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6469"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411805200"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961799n"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00097-1"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00124-6"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227471"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00037a042"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1110%2Fps.041255905","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1110\/ps.041255905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T22:38:33Z","timestamp":1697150313000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1110\/ps.041255905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1110\/ps.041255905"],"URL":"https:\/\/doi.org\/10.1110\/ps.041255905","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]}}}