{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:29:59Z","timestamp":1762673399266},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":1553,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The chaperone\u2010like activity of human lens \u03b1\u2010crystallin in inhibiting the aggregation of denatured proteins suggests a role for \u03b1\u2010crystallin in cataract prevention. Although a variety of techniques have generated structural information relevant to its chaperone\u2010like activity, the size and heterogeneity of \u03b1\u2010crystallin have prevented determination of its crystal structure. Even though synthetic cross\u2010linkers have provided considerable information about protein structures, they have not previously been used to study the proximity and orientation of subunits within human \u03b1\u2010crystallin. Cross\u2010linkers provide structural insight into proteins by binding the side chains of amino acids within close proximity. To identify the cross\u2010linked residues, the modified protein is digested and the resulting peptides are analyzed by mass spectrometry. Analysis of products from the reaction of \u03b1\u2010crystallin with 3,3\u2032dithiobis(sulfosuccinimidyl propionate), DTSSP, identified several modifications to both \u03b1A and \u03b1B. The most structurally informative of these modifications was a cross\u2010link between lysine 166 of \u03b1A and lysine 175 of \u03b1B. This cross\u2010link provides experimental evidence supporting theoretical structural models that place the C termini of \u03b1A and \u03b1B within close proximity in the native aggregate.<\/jats:p>","DOI":"10.1110\/ps.04910004","type":"journal-article","created":{"date-parts":[[2004,9,23]],"date-time":"2004-09-23T21:33:36Z","timestamp":1095975216000},"page":"2832-2835","source":"Crossref","is-referenced-by-count":7,"title":["The reaction of \u03b1\u2010crystallin with the cross\u2010linker 3,3\u2032\u2010dithiobis(sulfosuccinimidyl propionate) demonstrates close proximity of the C termini of \u03b1A and \u03b1B in the native assembly"],"prefix":"10.1002","volume":"13","author":[{"given":"Catherine L.","family":"Swaim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean B.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80180-1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.9.8.1503"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3380539"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1994.1101"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5475.2351"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(75)80286-9"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90204-8"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1565"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00002a015"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.03180004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10449"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(97)87625-0"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3242"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(77)80757-6"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.885"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(76)90011-5"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9824341"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1998.0482"},{"key":"e_1_2_5_20_1","doi-asserted-by":"crossref","first-page":"12494","DOI":"10.1016\/S0021-9258(18)99902-3","article-title":"Post\u2010translational modifications of water\u2010soluble human lens crystallins from young adults","volume":"269","author":"Miesbauer L.R.","year":"1994","journal-title":"J. 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