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Nonstructural protein 3 (NS3) is a multifunctional enzyme with serine protease and DEXH\/D\u2010box helicase domains, whose activity is central to flavivirus replication and is therefore a possible target for anti\u2010flaviviral compounds. Cloning, purification, and crystal structure determination to 1.9 \u00c5 resolution of the NS3 helicase of MVEV and characterization of its enzymatic activity is reported. Comparison with the structures of helicases from related viruses supports a possible mechanism of ATP hydrolysis\u2010driven strand separation.<\/jats:p>","DOI":"10.1110\/ps.072843107","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T21:03:05Z","timestamp":1190667785000},"page":"2294-2300","source":"Crossref","is-referenced-by-count":25,"title":["Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 \u00c5 resolution"],"prefix":"10.1002","volume":"16","author":[{"given":"Erika J.","family":"Mancini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"Assenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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