{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:39:44Z","timestamp":1787013584556,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,4,13]],"date-time":"2009-04-13T00:00:00Z","timestamp":1239580800000},"content-version":"vor","delay-in-days":2628,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The three\u2010dimensional structure of huwentoxin\u2010II (HWTX\u2010II), an insecticidal peptide purified from the venom of spider\n                    <jats:italic>Selenocosmia huwena<\/jats:italic>\n                    with a unique disulfide bond linkage as I\u2010III, II\u2010V, and IV\u2010VI, has been determined using 2D\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010NMR. The resulting structure of HWTX\u2010II contains two \u03b2\u2010turns (C4\u2010S7 and K24\u2010W27) and a double\u2010stranded antiparallel \u03b2\u2010sheet (W27\u2010C29 and C34\u2010K36). Although the C\u2010terminal double\u2010stranded \u03b2\u2010sheet cross\u2010linked by two disulfide bonds (II\u2010V and IV\u2010VI in HWTX\u2010II, II\u2010V and III\u2010VI in the ICK molecules) is conserved both in HWTX\u2010II and the ICK molecules, the structure of HWTX\u2010II is unexpected absence of the cystine knot because of its unique disulfide linkage. It suggests that HWTX\u2010II adopts a novel scaffold different from the ICK motif that is adopted by all other spider toxin structures elucidated thus far. Furthermore, the structure of HWTX\u2010II, which conforms to the disulfide\u2010directed \u03b2\u2010hairpin (DDH) motif, not only supports the hypothesis that the ICK is a minor elaboration of the more ancestral DDH motif but also suggests that HWTX\u2010II may have evolved from the same structural ancestor.\n                  <\/jats:p>","DOI":"10.1110\/ps.30502","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T19:59:58Z","timestamp":1027713598000},"page":"245-252","source":"Crossref","is-referenced-by-count":42,"title":["The structure of spider toxin huwentoxin\u2010II with unique disulfide linkage: Evidence for structural evolution"],"prefix":"10.1002","volume":"11","author":[{"given":"Qin","family":"Shu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010Yun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Cheng","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Ping","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm3.1989.370.1.485"},{"key":"e_1_2_7_3_1","first-page":"2059","article-title":"Solution structure of hpTX2, a toxin from Heteropodidae venatoria spider that blocks KV4.2 potassium channel","volume":"9","author":"Bernard C.","year":"2000","journal-title":"Protein Sci."},{"key":"e_1_2_7_4_1","volume-title":"X\u2010PLOR manual","author":"Br\u00fcnger A.T.","year":"1992"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0797-559"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(97)00301-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90392-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0406"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007011904904"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020663619657"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00149-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi952605r"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031022"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01452-X"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026314722607"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb1294-853"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00208803"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3011.1999.00039.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02109.x"},{"key":"e_1_2_7_21_1","first-page":"65","article-title":"Sequence\u2010specific assignment of 1H\u2010NMR resonance and determination of the secondary structure of HWTX\u2010II","volume":"33","author":"Shu Q.","year":"2001","journal-title":"Acta Biochim. 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