{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:39:11Z","timestamp":1790303951977,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":2890,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The repressor proteins of the LacI\/GalR family exhibit significant similarity in their secondary and tertiary structures despite less than 35% identity in their primary sequences. Furthermore, the core domains of these oligomeric repressors, which mediate dimerization, are homologous with the monomeric periplasmic binding proteins, extending the issue of plasticity to quaternary structure. To elucidate the determinants of assembly, a structure\u2010based alignment has been created for three repressors and four periplasmic binding proteins. Contact maps have also been constructed for the three repressor interfaces to distinguish any conserved interactions. These analyses show few strict requirements for assembly of the core N\u2010subdomain interface. The interfaces of repressor core C\u2010subdomains are well conserved at the structural level, and their primary sequences differ significantly from the monomeric periplasmic binding proteins at positions equivalent to LacI 281 and 282. However, previous biochemical and phenotypic analyses indicate that LacI tolerates many mutations at 281. Mutations at LacI 282 were shown to abrogate assembly, but for Y282D this could be compensated by a second\u2010site mutation in the core N\u2010subdomain at K84 to L or A. Using the link between LacI assembly and function, we have further identified 22 second\u2010site mutations that compensate the Y282D dimerization defect in vivo. The sites of these mutations fall into several structural regions, each of which may influence assembly by a different mechanism. Thus, the 360\u2010amino acid scaffold of LacI allows plasticity of its quaternary structure. The periplasmic binding proteins may require only minimal changes to facilitate oligomerization similar to the repressor proteins.<\/jats:p>","DOI":"10.1110\/ps.35801","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T00:00:34Z","timestamp":1027728034000},"page":"262-276","source":"Crossref","is-referenced-by-count":45,"title":["Plasticity of quaternary structure: Twenty\u2010two ways to form a LacI dimer"],"prefix":"10.1002","volume":"10","author":[{"given":"Liskin","family":"Swint\u2010Kruse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey R.","family":"Elam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer W.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane R.","family":"Wycuff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen Shive","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"57","article-title":"Dimer\u2010to\u2010tetramer assembly of Lac repressor involves a leucine heptad repeat","volume":"3","author":"Alberti S.","year":"1991","journal-title":"The New Biologist"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05992.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.15.4625-4630.1993"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi982577n"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/78907"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1785"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"22206","DOI":"10.1016\/S0021-9258(18)54555-5","article-title":"Characterization of mutations in oligomerization domain of lac repressor protien","volume":"266","author":"Chakerian A.E.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/S0021-9258(18)52302-4","article-title":"Evidence for leucine zipper motif in lactose repressor protein","volume":"266","author":"Chakerian A.E.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"17613","DOI":"10.1016\/S0021-9258(19)85376-0","article-title":"Lysine 84 is at the subunit interface of lac repressor protein","volume":"268","author":"Chang W.I.","year":"1993","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00178a018"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2571"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49645-2"},{"key":"e_1_2_7_15_1","first-page":"145","article-title":"T41 mutation in lac repressor is Tyr282 \u2192Asp","volume":"111","author":"Chen J.","year":"1992","journal-title":"Genes"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1598"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00367a019"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00367a018"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2902"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"3386","DOI":"10.1016\/S0021-9258(17)33449-X","article-title":"Limited proteolytic digestion of lac repressor by trypsin: Chemical nature of the resulting trypsin\u2010resistant core.","volume":"251","author":"Files J.G.","year":"1976","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7792597"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00624a020"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3706"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560071204"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5275.595"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/269668a0"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90659-T"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(90)90126-7"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5253.1247"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981617k"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1458"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)60035-5"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/73274"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3271"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)91033-L"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/302163a0"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","first-page":"13213","DOI":"10.1016\/S0021-9258(18)43029-3","article-title":"L\u2010Arabinose\u2010binding protein\u2010sugar complex at 2.4\u00c5 resolution","volume":"256","author":"Newcomer M.E.","year":"1981","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.30.18550"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"17602","DOI":"10.1016\/S0021-9258(19)85375-9","article-title":"Model of lactose repressor core based on alignment with sugar\u2010binding proteins is concordant with genetic and chemical data","volume":"268","author":"Nichols J.C.","year":"1993","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.12.5851"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/S0021-9258(19)44452-9","article-title":"Lac repressor: Specific proteolytic destruction of the NH2\u2010terminal region and loss of the deoxyribonucleic acid\u2010binding activity","volume":"248","author":"Platt T.","year":"1973","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/310381a0"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/310429a0"},{"key":"e_1_2_7_44_1","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1016\/S0021-9258(17)33445-2","article-title":"Mutations affecting the quaternary structure of the lac repressor","volume":"251","author":"Schmitz A.","year":"1976","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973627"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90243-0"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0356"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3469"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0479"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199811)20:11<884::AID-BIES3>3.0.CO;2-H"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.8.2427"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3057628"},{"key":"e_1_2_7_53_1","doi-asserted-by":"crossref","first-page":"5226","DOI":"10.1016\/S0021-9258(19)67776-8","article-title":"Comparison of the periplasmic receptors for L\u2010arabinose, D\u2010glucose\/D\u2010galactose, and D\u2010ribose: Structural and functional similarity","volume":"266","author":"Vyas N.K.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49615-4"},{"key":"e_1_2_7_55_1","volume-title":"Doctoral thesis","author":"Wycuff D.R.","year":"1999"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1110%2Fps.35801","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1110\/ps.35801","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T07:11:14Z","timestamp":1696662674000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1110\/ps.35801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1110\/ps.35801"],"URL":"https:\/\/doi.org\/10.1110\/ps.35801","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}