{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T18:00:11Z","timestamp":1789408811240,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":3652,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A photocrosslink between basic fibroblast growth factor (bFGF<jats:sub>155<\/jats:sub>) and a high affinity ssDNA oligonucleotide was characterized by positive ion electrospray ionization mass spectrometry (ESIMS). The DNA was a 61\u2010mer oligonucleotide photoaptamer bearing seven bromodeoxyuridines, identified by in vitro selection. Specific photocrosslinking of the protein to the oligonucleotide was achieved by 308 nm XeCl excimer laser excitation. The cross\u2010linked protein\u2010nucleic acid complex was proteolyzed with trypsin. The resulting peptide crosslink was purified by PAGE, eluted, and digested by snake venom phosphodiesterase\/alkaline phosphatase. Comparison of the oligonucleotide vs. the degraded peptide crosslink by high performance liquid chromatography coupled to an electrospray ionization triple quadrupole mass spectrometer showed a single ion unique to the crosslinked material. Sequencing by collision induced dissociation (MS\/MS) on a triple quadrupole mass spectrometer revealed that this ion was the nonapeptide TGQYKLGSK (residues 130\u2013138) crosslinked to a dinucleotide at Tyr133. The MS\/MS spectrum indicated sequential fragmentation of the oligonucleotide to uracil covalently attached to the nonapeptide followed by fragmentation of the peptide bonds. Tyr133 is located within the heparin binding pocket, suggesting that the in vitro selection targeted this negative ion binding region of bFGF<jats:sub>155<\/jats:sub>.<\/jats:p>","DOI":"10.1110\/ps.8.12.2806","type":"journal-article","created":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T21:50:02Z","timestamp":1234907402000},"page":"2806-2812","source":"Crossref","is-referenced-by-count":39,"title":["Mass spectral characterization of a protein\u2014nucleic acid photocrosslink"],"prefix":"10.1002","volume":"8","author":[{"given":"Mace C.","family":"Golden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katheryn A.","family":"Resing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tad H.","family":"Koch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael C.","family":"Willis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"6113","DOI":"10.1016\/S0021-9258(18)38091-8","article-title":"Identification of amino acids in lac re\u2010pressor protein cross\u2010linked to operator DNA specifically substituted with bromodeoxyuridine","volume":"266","author":"Allen T","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_3_1","first-page":"239","article-title":"The fibroblast growth factor family","volume":"3","author":"Baird A","year":"1991","journal-title":"Cancer Cells"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"21870","DOI":"10.1016\/S0021-9258(17)31883-5","article-title":"UV\u2010catalyzed cross\u2010linking of Escherichia coli uracil\u2010DNA glycosylase to DNA","volume":"269","author":"Bennett SE","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)93460-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)93433-L"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1998.tb03244.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1998.tb09685.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1987.tb04879.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1989.tb04085.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5252.1116"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00239a030"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00177a030"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81720-1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12220"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290070619"},{"key":"e_1_2_1_17_1","first-page":"27","volume-title":"Techniques in protein chemistry V","author":"Jensen ON","year":"1994"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.19.3866"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00078a054"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409239709108550"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","unstructured":"MeisenheimerKM MeisenheimerPL KochTH.1999.Nucleoprotein photocrosslinking using halopyrimidine\u2010substituted RNAs.Methods Enzymol. 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