{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:23:52Z","timestamp":1789892632807,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":3287,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS\u2010stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop\u2010cleaved crmA to 2.9 \u00c5 resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for \u03b2 sheet A, whose sequence and length are unique to crmA.<\/jats:p>","DOI":"10.1110\/ps.9.8.1423","type":"journal-article","created":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T23:25:15Z","timestamp":1234913115000},"page":"1423-1427","source":"Crossref","is-referenced-by-count":37,"title":["Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition"],"prefix":"10.1002","volume":"9","author":[{"given":"Miljan","family":"Simonovic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PETER G. W.","family":"Gettins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Volz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90704-A"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)91062-T"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444998003254"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8303301"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.49.32608"},{"key":"e_1_2_1_7_1","volume-title":"Serpins: Structure, function and biology","author":"Gettins PGW","year":"1996"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(85)15021-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00449a001"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889891004430"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00009-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(94)00224-5"},{"key":"e_1_2_1_13_1","first-page":"731","article-title":"Human \u03b11\u2010proteinase inhibitor. Crystal structure analysis of two crystal modifications and preliminary analysis of the implications for function","volume":"177","author":"L\u00f6bermann H","year":"1984","journal-title":"J Mol Biol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1378"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340110407"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76072-5"},{"key":"e_1_2_1_17_1","volume-title":"QUANTA basic operations","year":"1997"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90223-Y"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","unstructured":"SimonovicM GettinsPGW VolzK.2000. Crystallization and preliminary X\u2010ray diffraction analysis of a recombinant cysteine\u2010free mutant of crmA.J Acta Crystallogr Sect D. 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