{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:25:43Z","timestamp":1771302343172,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"Abstract<\/jats:title>Epidermolysis bullosa acquisita (EBA<\/jats:styled-content>) is an autoimmune subepidermal blistering disease caused by autoantibodies against type VII<\/jats:styled-content> collagen. An enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) is currently available to detect autoantibodies in EBA<\/jats:styled-content>. There have been reports suggesting generically that ELISA<\/jats:styled-content> indices reflect EBA<\/jats:styled-content> disease severity; however, there is, as yet, no conclusion as to whether ELISA<\/jats:styled-content> indices fluctuate with disease activity over time in each EBA<\/jats:styled-content> patient. This study aimed to investigate whether ELISA<\/jats:styled-content> titers fluctuate with EBA<\/jats:styled-content> disease activity and to validate the clinical significance of checking ELISA<\/jats:styled-content> values in EBA<\/jats:styled-content> by monitoring type VII<\/jats:styled-content> collagen ELISA<\/jats:styled-content> titers and disease severity, evaluated in terms of numbers of blisters and erosions as a clinical score, over time in three J<\/jats:styled-content>apanese patients with EBA<\/jats:styled-content>. All three cases in this study, which were treated successfully, showed titers of anti\u2010type VII<\/jats:styled-content> collagen autoantibodies detected by ELISA<\/jats:styled-content> that fluctuated in parallel with disease activity. Especially in case 1, we could determine that the expanding erosions were not due to flare\u2010ups of EBA<\/jats:styled-content> because the ELISA<\/jats:styled-content> indices stayed low, although new lesions continued to appear. In fact, control of infection and nutrition helped the lesions to become epithelialized. In conclusion, we found that repeated ELISA<\/jats:styled-content> measurements are useful in monitoring disease activity and making decisions in EBA<\/jats:styled-content> treatment plans.<\/jats:p>","DOI":"10.1111\/1346-8138.12278","type":"journal-article","created":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T10:03:12Z","timestamp":1381917792000},"page":"864-868","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010type VII<\/scp> collagen autoantibodies, detected by enzyme\u2010linked immunosorbent assay, fluctuate in parallel with clinical severity in patients with epidermolysis bullosa acquisita"],"prefix":"10.1111","volume":"40","author":[{"given":"Yoshihiro","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Kasai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Keio 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