{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T13:39:03Z","timestamp":1768311543886,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T00:00:00Z","timestamp":1379894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The relationship of ovostatin 2 (<jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2) expression with the clinicopathological features of cutaneous malignant melanoma (<jats:styled-content style=\"fixed-case\">CMM<\/jats:styled-content>) was investigated to identify <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 expression in cutaneous melanocytic lesions, and to reveal whether <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 has a function in melanoma progression. Eight specimens of <jats:styled-content style=\"fixed-case\">CMM<\/jats:styled-content> and paracancerous tissue were analyzed using real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and western blot for the m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein expression of <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2, respectively. Immunohistochemical staining was performed on 52 <jats:styled-content style=\"fixed-case\">CMM<\/jats:styled-content> and 62 nevi, followed by clinicopathological significance analysis. The proliferative cells were visualized by staining with Ki\u201067 antibody. The intensity of angiogenesis was assessed by staining with vascular endothelial growth factor (<jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>). Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and western blot analyses showed that <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 was significantly upregulated in cutaneous melanoma than in paired normal skins. Immunohistochemistry showed that 86.5% (45\/52) of malignant cases showed <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 cytoplasmic expression compared with 29% (18\/62) in benign nevi. OVOS2 expression was significantly higher in invasive and metastatic melanoma than in <jats:italic>in situ<\/jats:italic> melanoma (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01). Furthermore, <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 expression was positively correlated with the known prognostic variables of melanoma including clinical stage, <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lark level and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>reslow depth. It was also significantly associated with ulcer status, Ki\u201067 labeling index and <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> expression in primary melanoma. <jats:styled-content style=\"fixed-case\">OVOS<\/jats:styled-content>2 expression was significantly increased in <jats:styled-content style=\"fixed-case\">CMM<\/jats:styled-content>, which increased incrementally from benign nevi to melanoma and appeared to be involved in the progression of melanoma.<\/jats:p>","DOI":"10.1111\/1346-8138.12294","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:01:15Z","timestamp":1381528875000},"page":"901-910","source":"Crossref","is-referenced-by-count":5,"title":["Expression analysis of ovostatin 2 reveals its involvement in proliferation, invasion and angiogenesis of cutaneous malignant melanoma"],"prefix":"10.1111","volume":"40","author":[{"given":"Ying\u2010Xue","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Biology for Skin Diseases Institute of Dermatology Chinese Academy of Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinliang","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Sheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biology for Skin Diseases Institute of Dermatology Chinese Academy of Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Bao","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biology for Skin Diseases Institute of Dermatology Chinese Academy 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