{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T13:44:46Z","timestamp":1778939086792,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,12,4]],"date-time":"2013-12-04T00:00:00Z","timestamp":1386115200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Loop\u2010mediated isothermal amplification (<jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>) is a nucleic acid amplification method with a high specificity, efficiency and speed. No reports exist regarding the usefulness of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> for clinically suspected skin infections caused by herpes simplex virus (<jats:styled-content style=\"fixed-case\">HSV<\/jats:styled-content>) or varicella zoster virus (<jats:styled-content style=\"fixed-case\">VZV<\/jats:styled-content>). The aim of this study was to evaluate the clinical usefulness of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> in the diagnosis of common cutaneous alpha herpesvirus (<jats:styled-content style=\"fixed-case\">HSV<\/jats:styled-content> type 1 and 2, and <jats:styled-content style=\"fixed-case\">VZV<\/jats:styled-content>) infections. <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> and real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) were performed using swab samples collected from 106 patients with clinically suspected alpha herpesvirus skin infections. The results of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> performed with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction did not differ from those performed without <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction. The sensitivity of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> tested against real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was 96% in herpes simplex, 78% in eczema herpeticum, 93% in herpes zoster and 100% in varicella. No viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was detected by <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> in all negative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> samples. Viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> load was significantly lower in samples with false\u2010negative <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> results than in the <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>\u2010positive samples. <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> enables confirmation of clinically suspected cutaneous <jats:styled-content style=\"fixed-case\">HSV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">VZV<\/jats:styled-content> infections. However, the sensitivity of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> is lower than real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The accuracy of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> may increase if sufficient viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> is obtained from lesions. <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> performed without <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction remains sensitive; thus, <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> represents a quick and economical method for the diagnosis of common alpha herpesvirus skin infections.<\/jats:p>","DOI":"10.1111\/1346-8138.12325","type":"journal-article","created":{"date-parts":[[2013,12,4]],"date-time":"2013-12-04T13:10:32Z","timestamp":1386162632000},"page":"1033-1037","source":"Crossref","is-referenced-by-count":16,"title":["Clinical utility of loop\u2010mediated isothermal amplification assay for the diagnosis of common alpha herpesvirus skin infections"],"prefix":"10.1111","volume":"40","author":[{"given":"Tsukane","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Yagami","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayoko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kariya Toyota General Hospital Kariya Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Ihira","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsushi","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayoko","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195248"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199703000-00010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199806)55:2<177::AID-JMV15>3.0.CO;2-F"},{"issue":"2","key":"e_1_2_6_5_1","first-page":"61","article-title":"Comparison of virus isolation and various 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