{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:47:51Z","timestamp":1757314071963},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T00:00:00Z","timestamp":1388707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Abstract<\/jats:title>Matrix\u2010assisted laser desorption and ionization time\u2010of\u2010flight mass spectrometry (MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\u2010MS<\/jats:styled-content>) has been utilized for identification of various microorganisms. M<\/jats:styled-content>alassezia<\/jats:italic> species, including M<\/jats:styled-content>alassezia restricta<\/jats:italic>, which is associated with seborrheic dermatitis, has been difficult to identify by traditional means. This study was performed to develop a system for identification of M<\/jats:styled-content>alassezia<\/jats:italic> species with MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\u2010MS<\/jats:styled-content> and to investigate the incidence and variety of cutaneous M<\/jats:styled-content>alassezia<\/jats:italic> microbiota of 1\u2010month\u2010old infants using this technique. A M<\/jats:styled-content>alassezia<\/jats:italic> species\u2010specific MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\u2010MS<\/jats:styled-content> database was developed from eight standard strains, and the availability of this system was assessed using 54 clinical strains isolated from the skin of 1\u2010month\u2010old infants. Clinical isolates were cultured initially on CHROMagar M<\/jats:styled-content>alassezia<\/jats:italic> growth medium, and the 28S ribosomal DNA<\/jats:styled-content> (D1\/D2) sequence was analyzed for confirmatory identification. Using this database, we detected and analyzed M<\/jats:styled-content>alassezia<\/jats:italic> species in 68% and 44% of infants with and without infantile seborrheic dermatitis, respectively. The results of MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\u2010MS<\/jats:styled-content> analysis were consistent with those of rDNA<\/jats:styled-content> sequencing identification (100% accuracy rate). To our knowledge, this is the first report of a MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\u2010MS<\/jats:styled-content> database for major skin pathogenic M<\/jats:styled-content>alassezia<\/jats:italic> species. This system is an easy, rapid and reliable method for identification of M<\/jats:styled-content>alassezia<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1346-8138.12364","type":"journal-article","created":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T13:08:20Z","timestamp":1388754500000},"page":"117-123","source":"Crossref","is-referenced-by-count":13,"title":["Utilization of matrix\u2010assisted laser desorption and ionization time\u2010of\u2010flight mass spectrometry for identification of infantile seborrheic dermatitis\u2010causing M<\/scp>alassezia<\/i> and incidence of culture\u2010based cutaneous M<\/scp>alassezia<\/i> microbiota of 1\u2010month\u2010old infants"],"prefix":"10.1111","volume":"41","author":[{"given":"Mikachi","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Laboratory of Space and Environmental Medicine Graduate School of Medicine Teikyo University Tokyo 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