{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:42:56Z","timestamp":1778924576873,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T00:00:00Z","timestamp":1397865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Calcitriol had been proved to be effective for treatment of psoriasis vulgaris. However, the molecular events leading to the normalization of keratinocyte differentiation had not been fully explored. The aim of the study was to evaluate the role of calcitriol and serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 in human keratinocyte cell line (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>) proliferation. Proteins extracted from calcitriol\u2010treated and untreated <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> were separated by 2\u2010D differential gel electrophoresis (2<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>). Then, the 2<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content> profiles were analyzed to screen for differentially expressed proteins, which were identified by matrix\u2010assisted laser desorption\/ionization time of flight mass spectrometry. The upregulation of serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 was confirmed by quantitative reverse transcription polymerase chain reaction (q<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and western blot analysis. The effect of serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 on <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> proliferation was analyzed by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference experiments, methylthiazoletetrazolium assay and flow cytometry. Reproducible 2\u2010<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content> profiles of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> were established. The result that serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 was upregulated in the calcitriol\u2010treated group was confirmed by q<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and western blot analysis. Calcitriol could inhibit proliferation of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> at the concentration of 10<jats:sup>\u22129<\/jats:sup>\u201310<jats:sup>\u22126\u00a0<\/jats:sup>mol\/L. <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> proliferation was promoted when serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 was interfered. The inhibition effect of calcitriol was stopped after serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 was interfered. Serpin <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>1 was overexpressed in calcitriol\u2010treated <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells and may play an important role in inhibiting <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> proliferation by calcitriol.<\/jats:p>","DOI":"10.1111\/1346-8138.12434","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T11:19:59Z","timestamp":1397906399000},"page":"393-398","source":"Crossref","is-referenced-by-count":9,"title":["Calcitriol inhibits keratinocyte proliferation by upregulating leukocyte elastase inhibitor (serpin B1)"],"prefix":"10.1111","volume":"41","author":[{"given":"Li","family":"Hui","sequence":"first","affiliation":[{"name":"Department of Dermatology Changhai Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Yongxia","sequence":"additional","affiliation":[{"name":"Medical College of Jiaxing University Jiaxing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo","family":"Zhili","sequence":"additional","affiliation":[{"name":"Department of Dermatology Changhai Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gu","family":"Jun","sequence":"additional","affiliation":[{"name":"Department of Dermatology Changhai Hospital Second Military Medical University Shanghai 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