{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:07:05Z","timestamp":1791328025380,"version":"4.3.1"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T00:00:00Z","timestamp":1427328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"the Ministry of Education, Culture, Sports, Science and Technology in Japan.","doi-asserted-by":"crossref","award":["22791057"],"award-info":[{"award-number":["22791057"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Abstract<\/jats:title>CCR<\/jats:styled-content>4 is expressed on tumor cells of mycosis fungoides (MF<\/jats:styled-content>) and S\u00e9zary syndrome (SS<\/jats:styled-content>). In MF<\/jats:styled-content>, most infiltrating cells in patches and plaques express CXCR<\/jats:styled-content>3, while tumor cells express CCR<\/jats:styled-content>4 in advanced stages. Poteligeo Test IHC<\/jats:styled-content> (CCR<\/jats:styled-content>4 staining kit) is a newly developed staining kit that can examine the presence of CCR<\/jats:styled-content>4 expressed on tumor cells of adult T\u2010cell leukemia\/lymphoma, peripheral T\u2010cell lymphoma and cutaneous T\u2010cell lymphoma before treatment of anti\u2010CCR<\/jats:styled-content>4 antibody using paraffin\u2010embedded samples. In this study, we analyzed CCR<\/jats:styled-content>4 expression in lesional skin of MF<\/jats:styled-content>, SS<\/jats:styled-content>, atopic dermatitis (AD<\/jats:styled-content>) and psoriasis with this new kit. CCR<\/jats:styled-content>4 was expressed on infiltrating cells in lesional skin of patch, plaque, tumor MF<\/jats:styled-content> and SS<\/jats:styled-content>, and the number of positive cells increased as the disease progressed. Immunohistochemistry with frozen sections also showed some positive cells scattered in the dermis, although the quality was not high enough to quantify positive cells. There were significant positive correlations between CCR<\/jats:styled-content>4+<\/jats:sup> cells and serum lactate dehydrogenase levels. Interestingly, CCR<\/jats:styled-content>4+<\/jats:sup> cells were also detected in AD<\/jats:styled-content> skin, whose number was larger than that in psoriatic skin. Previous studies showed only scattered CCR<\/jats:styled-content>4+<\/jats:sup> cells in skin samples by standard immunohistochemical staining. The new, sensitive CCR<\/jats:styled-content>4 staining kit has revealed that CCR<\/jats:styled-content>4 is expressed on infiltrating cells in lesional skin of early MF<\/jats:styled-content> and AD<\/jats:styled-content> as well as advanced MF<\/jats:styled-content> and SS<\/jats:styled-content>. These cells can be therapeutic targets for patients who are resistant to standard treatments.<\/jats:p>","DOI":"10.1111\/1346-8138.12852","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T19:59:43Z","timestamp":1427399983000},"page":"613-615","source":"Crossref","is-referenced-by-count":45,"title":["CCR<\/scp>4 is expressed on infiltrating cells in lesional skin of early mycosis fungoides and atopic dermatitis"],"prefix":"10.1111","volume":"42","author":[{"given":"Makoto","family":"Sugaya","sequence":"first","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohshi","family":"Morimura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiraku","family":"Suga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makiko","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomitsu","family":"Miyagaki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanako","family":"Ohmatsu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2006.06.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2003.132"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2010.05.005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12555.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.07039.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1505"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-2697"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2004.06325.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-014-1501-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.054"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1346-8138.12852","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.12852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:30:42Z","timestamp":1695504642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.12852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,26]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1111\/1346-8138.12852"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.12852","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,26]]}}}