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To establish an easy and accurate diagnosis of the eight disease groups, we constructed recombinant adenoviruses that expressed one of the <jats:italic><jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content><\/jats:italic> <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>. When fibroblasts derived from patients with <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010A, \u2010B, \u2010C, \u2010D, \u2010F or \u2010G were infected with the adenovirus expressing <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> XPB<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> XPC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> XPD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> XPF<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">XPG<\/jats:styled-content>, respectively, and <jats:styled-content style=\"fixed-case\">UV\u2010C<\/jats:styled-content> at 5\u201320 J\/m<jats:sup>2<\/jats:sup> was irradiated, cell viability was clearly recovered by the corresponding recombinant adenoviruses. In contrast, <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010E and <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010V cells were not significantly sensitive to <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> irradiation and were barely complemented by the matched recombinant adenoviruses. However, co\u2010infection of Ad\u2010<jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content> with Ad\u2010<jats:styled-content style=\"fixed-case\">XPE<\/jats:styled-content> increased survival rate of <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010E cells after <jats:styled-content style=\"fixed-case\">UV\u2010C<\/jats:styled-content> exposure. When <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010V cell strains, including one derived from a Japanese patient, were infected with Ad\u2010<jats:styled-content style=\"fixed-case\">XPV<\/jats:styled-content>, exposed to <jats:styled-content style=\"fixed-case\">UV\u2010B<\/jats:styled-content> and cultured with 1 mmol\/L of caffeine, flow cytometry detected a characteristic decrease in the S phase in all the <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010V cell strains. From these results, the eight groups of <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content> could be differentiated by utilizing a set of recombinant adenoviruses, indicating that our procedure provides a convenient and correct diagnostic method for all the <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content> groups including <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010E and <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content>\u2010V.<\/jats:p>","DOI":"10.1111\/1346-8138.13333","type":"journal-article","created":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T06:34:22Z","timestamp":1457937262000},"page":"1167-1173","source":"Crossref","is-referenced-by-count":3,"title":["Diagnosis of eight groups of xeroderma pigmentosum by genetic complementation using recombinant adenovirus vectors"],"prefix":"10.1111","volume":"43","author":[{"given":"Toshiharu","family":"Yamashita","sequence":"first","affiliation":[{"name":"Department of Dermatology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masae","family":"Okura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasue","family":"Ishii\u2010Osai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tokimasa","family":"Hida","sequence":"additional","affiliation":[{"name":"Department of Dermatology Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.426"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/218652a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1001\/jama.1969.03150240093011","article-title":"Xeroderma pigmentosum. 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