{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:36:45Z","timestamp":1764603405677},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T00:00:00Z","timestamp":1471651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2012R1A1A2042581"],"award-info":[{"award-number":["2012R1A1A2042581"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anagen effluvium develops because of disturbances in the hair follicle cycle, leading to acute and severe hair loss in humans. The objective of this study was to establish a mouse model of anagen effluvium by 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) treatment, and evaluate the pathological changes and underlying mechanisms. We treated 9\u201310\u2010day\u2010old pups and 3\u20137\u2010week\u2010old C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice with BrdU. After successfully inducing hair loss in the neonatal pups, microscopic, immunohistochemical and flow cytometry analyses were conducted. BrdU induced early onset alopecia in neonates and caused epidermal thickening and hair shaft breakage. BrdU appeared to incorporate the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>326\u2010positive keratinocyte layer and induced p53\u2010related apoptosis. Keratinocyte apoptosis caused immune cell infiltration in the dermal region; M2 macrophages and neutrophils were dominant. The BrdU\u2010induced hair loss was dose\u2010dependent, and alopecia was visible at a dose range of 25\u2013200 \u03bcg\/g bodyweight. The BrdU\u2010induced anagen effluvium mouse model is novel and easily established by administrating four simple BrdU injections to pups; these mice showed synchronized onset of alopecia symptoms with little individual variation. Moreover, this model showed an alopecia phenotype similar to that of human anagen effluvium with acute, severe and widespread hair loss.<\/jats:p>","DOI":"10.1111\/1346-8138.13547","type":"journal-article","created":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T11:26:49Z","timestamp":1471692409000},"page":"180-185","source":"Crossref","is-referenced-by-count":1,"title":["5\u2010Bromo\u20102\u2032\u2010deoxyuridine induced effluvium via p53\u2010mediated <scp>CD<\/scp>326\u2010positive keratinocyte apoptosis in C57<scp>BL<\/scp>\/6 mice"],"prefix":"10.1111","volume":"44","author":[{"given":"Kyung\u2010Jun","family":"Uh","sequence":"first","affiliation":[{"name":"Designed Animal and Transplantation Research Institute Institute of Greenbio Research and Technology Seoul National University Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo\u2010Sung","family":"Hwang","sequence":"additional","affiliation":[{"name":"Designed Animal and Transplantation Research Institute Institute of Greenbio Research and Technology Seoul National University Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hyun","family":"Bae","sequence":"additional","affiliation":[{"name":"Designed Animal and Transplantation Research Institute Institute of Greenbio Research and Technology Seoul National University Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Eun","family":"Jang","sequence":"additional","affiliation":[{"name":"Graduate School of International Agricultural Technology Seoul National University Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Cheong","family":"Yeom","sequence":"additional","affiliation":[{"name":"Graduate School of International Agricultural Technology Seoul National University Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2003.05565.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1087-0024.2005.10102.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-0938-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.116728"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00140.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/07357909209032751"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23787.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1213-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-0712-x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.07.004"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.01377.x"},{"issue":"4","key":"e_1_2_7_13_1","first-page":"719","article-title":"Chemotherapy\u2010induced alopecia in mice. 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