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The aim of this study was to analyze the utility of fluorescence <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) in the detection of the collagen type I\u2010\u03b11\/platelet derived growth factor\u2010\u03b2 (<jats:italic><jats:styled-content style=\"fixed-case\">COL<\/jats:styled-content>1A1\u2013<jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic>) fusion gene in biopsies of <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content>. Twenty\u2010three consecutive biopsy specimens of <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content> were reviewed for clinicopathological features and examined with the <jats:italic><jats:styled-content style=\"fixed-case\">COL<\/jats:styled-content>1A1\u2013<jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic> fusion probe and <jats:italic><jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic> break\u2010apart probe using <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> analysis. The 23 tumor samples consisted of 11 males and 12 females (mean age at diagnosis, 37\u00a0years; range, 14\u201375 years). Eighteen conventional <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content>, one Bednar tumor, two myxoid <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content> and two fibrosarcomatous <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content> samples were included in the group. Strong and extensive <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 expression was observed in 19 of 23 cases (83%). Twenty\u2010one cases (91%) were positive for both the <jats:italic><jats:styled-content style=\"fixed-case\">COL<\/jats:styled-content>1A1\u2013<jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic> fusion signal and the <jats:italic><jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic> break\u2010apart signal. This is one of the few studies to demonstrate the value of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> analysis of the <jats:italic><jats:styled-content style=\"fixed-case\">COL<\/jats:styled-content>1A1\u2013<jats:styled-content style=\"fixed-case\">PDGFB<\/jats:styled-content><\/jats:italic> gene, which could validate complicated and suspected diagnoses in the routine biopsy of <jats:styled-content style=\"fixed-case\">DFSP<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/1346-8138.13767","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T07:11:29Z","timestamp":1486019489000},"page":"798-802","source":"Crossref","is-referenced-by-count":17,"title":["Application of <i><scp>COL<\/scp>1A1\u2013<scp>PDGFB<\/scp><\/i> fusion gene detection by fluorescence <i>in\u00a0situ<\/i> hybridization in biopsy tissue of dermatofibrosarcoma protuberans"],"prefix":"10.1111","volume":"44","author":[{"given":"Zhang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"77","volume-title":"World Health Organization classification of soft tissue and bone tumours","author":"Mentzel T","year":"2013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000134178"},{"key":"e_1_2_7_4_1","first-page":"1553","article-title":"Ring chromosomes in dermatofibrosarcoma protuberans are composed of interspersed sequences from chromosomes 17 and 22","volume":"147","author":"Naeem R","year":"1995","journal-title":"Am J 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