{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T09:35:44Z","timestamp":1771493744583,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T00:00:00Z","timestamp":1490918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japanese Ministry of Health, Labor and Welfare"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have indicated that various nucleic acids are present in human sera, and attracted attention for their potential as novel disease markers in many human diseases. In this study, we tried to evaluate the possibility that DNA and RNA of collagens exist in human sera, and determined whether their serum levels can be useful biomarkers in scleroderma patients. The RNA or DNA of collagens were purified from sera, and detected by polymerase chain reaction or quantitated by real\u2010time polymerase chain reaction. Among approximately 18 360 bases of full\u2010length \u03b11(I) collagen DNA, various regions were detected by polymerase chain reaction in human sera. However, \u03b12(I) collagen DNA, \u03b11(I) collagen RNA or \u03b12(I) collagen RNA were not detectable. \u03b11(I) Collagen DNA in sera was quantitative using our method. The levels of serum \u03b11(I) collagen DNA were significantly increased in scleroderma patients compared with healthy control subjects or systemic lupus erythematosus patients. According to the receiver\u2013operator curve analysis, serum \u03b11(I) collagen DNA levels were shown to be effective as a diagnostic marker of scleroderma. Furthermore, when we determined the association of serum \u03b11(I) collagen DNA levels with clinical\/laboratory features in scleroderma patients, those with elevated \u03b11(I) collagen DNA levels showed significantly higher prevalence of pitting scars\/ulcers. In summary, elevation of serum \u03b11(I) collagen DNA levels in scleroderma patients may be useful as the diagnostic marker, reflecting the presence of vasculopathy.<\/jats:p>","DOI":"10.1111\/1346-8138.13838","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T07:07:25Z","timestamp":1490944045000},"page":"927-931","source":"Crossref","is-referenced-by-count":3,"title":["Serum levels of genomic DNA of \u03b11(I) collagen are elevated in scleroderma patients"],"prefix":"10.1111","volume":"44","author":[{"given":"Soichiro","family":"Sawamura","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9230-6207","authenticated-orcid":false,"given":"Masatoshi","family":"Jinnin","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery 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