{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:36:04Z","timestamp":1789968964702,"version":"4.0.1"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,6,17]],"date-time":"2017-06-17T00:00:00Z","timestamp":1497657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prevalence of antimicrobial\u2010resistant<jats:italic>Propionibacterium acnes<\/jats:italic>strains isolated from acne patients has been increasing in Japan. Here, to estimate the current resistance rate, we tested antimicrobial susceptibility among<jats:italic>P. acnes<\/jats:italic>from acne patients having visited a specialized dermatology clinic between 2013 and 2015. Rates of resistance to macrolides and clindamycin were 44.3 (31\/70) and 38.6% (27\/70), respectively.<jats:italic>erm<\/jats:italic>(X), which confers high\u2010level clindamycin resistance (minimum inhibitory concentration \u2265256 \u03bcg\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>), was detected in six isolates, whereas no resistance determinants were identified in eight strains showing high\u2010level resistance to clindamycin. Using single\u2010locus sequence typing, the<jats:italic>P. acnes<\/jats:italic>isolates were classified into five clades (A, E, F, H and K), with all high\u2010level clindamycin\u2010resistant strains lacking known clindamycin resistance determinants being grouped together (in clade F).<jats:italic>P. acnes<\/jats:italic>isolates from patients previously treated with macrolides and clindamycin showed a macrolide resistance rate (55.3%) significantly higher than that of those from patients not having received these treatments (21.7%,<jats:italic>P<\/jats:italic>&lt; 0.05). Furthermore, strains of clade F, which were very rarely isolated from healthy individuals, were more frequently recovered from patients with severe acne (40.0%) than those with mild acne (23.3%). Our data showed an increase in macrolide\u2010resistant<jats:italic>P. acnes<\/jats:italic>prevalence in Japan due to the use of antimicrobial agents for acne treatment. Furthermore, we identified strains of specific phylogenetic groups frequently associated with severe acne patients.<\/jats:p>","DOI":"10.1111\/1346-8138.13913","type":"journal-article","created":{"date-parts":[[2017,6,17]],"date-time":"2017-06-17T23:24:52Z","timestamp":1497741892000},"page":"1248-1254","source":"Crossref","is-referenced-by-count":67,"title":["Antimicrobial susceptibility and phylogenetic analysis of<i>Propionibacterium acnes<\/i>isolated from acne patients in Japan between 2013 and 2015"],"prefix":"10.1111","volume":"44","author":[{"given":"Keisuke","family":"Nakase","sequence":"first","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobukazu","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Toranomon Hospital Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yui","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sae","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-7297","authenticated-orcid":false,"given":"Norihisa","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1171700"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2014.02.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104199"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13027-016-0084-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9397"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12594"},{"key":"e_1_2_7_8_1","first-page":"1893","article-title":"A Acne Vulgaris Treatment Guideline","volume":"118","author":"Hayashi N","year":"2008","journal-title":"Jpn J Dermatol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2003.05067.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2005.01.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00081.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2011.01423.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.067611-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.1.165"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2016.05.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04690.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2016.10.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012277"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1346-8138.2007.00403.x-i1","article-title":"Acne Study G. Establishment of grading criteria for acne severity","volume":"35","author":"Hayashi N","year":"2008","journal-title":"J Dermatol"},{"key":"e_1_2_7_20_1","unstructured":"CLSI.National Committee for Clinical Laboratory Standards. Methods of Antimicrobial Susceptibility Testing of Anaerobic Bacteria\u2010Eighth Edition; Approved Standard M11\u2010A8.2012."},{"key":"e_1_2_7_21_1","unstructured":"CLSI.Methods for Dilution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically; Approved standard. 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