{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T10:38:21Z","timestamp":1766486301679,"version":"3.37.3"},"reference-count":7,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T00:00:00Z","timestamp":1501718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Mycobacterium haemophilum<\/jats:italic> is a slow\u2010growing, non\u2010tuberculous mycobacteria that causes cutaneous infection. We describe a case of cutaneous infection in a 68\u2010year\u2010old Japanese man with polymyositis. This was caused by <jats:italic>M. haemophilum<\/jats:italic> harboring one base insertion in gene sequence. At first, the causal microorganism was misidentified as <jats:italic>M. intracellulare by <\/jats:italic><jats:styled-content style=\"fixed-case\">COBAS<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup> TaqMan<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">MAI<\/jats:styled-content> test. However, poor growth on Ogawa media and growth enhancement on 7H11C agar around a hemin\u2010containing disk prompted us to reinvestigate the causal microorganisms, which were revealed to be <jats:italic>M. haemophilum<\/jats:italic>. Amplified polymerase chain reaction products were sequenced, and the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene, <jats:italic>rpoB<\/jats:italic>,<jats:italic> hsp65<\/jats:italic> and internal transcribed spacer region sequences showed a 100%, 100%, 99.66% and 99.7% match, respectively, with the corresponding regions of <jats:italic>M. haemophilum<\/jats:italic>, but it harbored a novel gene sequence in <jats:italic>hsp65<\/jats:italic>. The sequences determined by gene analysis of the <jats:italic>M. haemophilum<\/jats:italic> strain were deposited into the International Nucleotide Sequence Database. Although numerous cases of <jats:italic>M. haemophilum<\/jats:italic> infection have been reported in other countries, only six cases have been reported in Japan to date. It could be possible that this novel mutation lead to misdiagnosis. As <jats:italic>M. haemophilum<\/jats:italic> prefers a lower growth temperature (30\u201332\u00b0C) and it requires iron in the culture medium, <jats:italic>M. haemophilum<\/jats:italic> could be misidentified or overlooked. Accordingly, a <jats:italic>M. haemophilum<\/jats:italic> infection should be considered in cases of cutaneous infection of the body sites, of which surface temperature is low.<\/jats:p>","DOI":"10.1111\/1346-8138.13988","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T19:38:02Z","timestamp":1501789082000},"page":"64-66","source":"Crossref","is-referenced-by-count":6,"title":["Case of <i>Mycobacterium haemophilum<\/i> misdiagnosed as <i>Mycobacterium intracellulare<\/i> due to one base insertion in the bacterial genome"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3719-5035","authenticated-orcid":false,"given":"Rika","family":"Nishikawa","sequence":"first","affiliation":[{"name":"Dermatology Department of Internal Related Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yozo","family":"Yamada","sequence":"additional","affiliation":[{"name":"Dermatology Department of Internal Related Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruhisa","family":"Kanki","sequence":"additional","affiliation":[{"name":"Dermatology Department of Internal Related Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Division of Oncology\/Hematology Department of Internal Medicine Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kobe University Hospital Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Jikimoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kobe University Hospital Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Kusuki","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kobe University Hospital Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1566-0031","authenticated-orcid":false,"given":"Norihisa","family":"Ishii","sequence":"additional","affiliation":[{"name":"Leprosy Research Center National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Ohnuma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Kobe University Hospital Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazue","family":"Nakanaga","sequence":"additional","affiliation":[{"name":"Leprosy Research Center National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6784-2849","authenticated-orcid":false,"given":"Chikako","family":"Nishigori","sequence":"additional","affiliation":[{"name":"Dermatology Department of Internal Related Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.11164.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2012.01533.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05088-11"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.12047"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s101560100033"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.11150\/kansenshogakuzasshi1970.78.389"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02798.x"}],"container-title":["The Journal of 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