{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:14:44Z","timestamp":1785410084979,"version":"3.56.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T00:00:00Z","timestamp":1551052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Matching Fund Subsidy for the Private Schools of Japan"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antimicrobial agents have been used for eradication of <jats:italic>Cutibacterium<\/jats:italic> (formerly <jats:italic>Propionibacterium<\/jats:italic>) <jats:italic>acnes<\/jats:italic> that is an exacerbation factor of the skin disease acne vulgaris. However, the use of antibiotics is associated with an increased risk of promoting the emergence of resistant bacteria and leading to skin dysbiosis. Traditional Japanese Kampo medicines, such as Keigairengyoto, are used to treat acne. However, there is incomplete understanding regarding their functional mechanism in treatment of acne. In this study, we examined the antimicrobial and anti\u2010lipase activity of the Kampo medicines used empirically for acne treatment. Three oral medicines, Keigairengyoto, Seijoboufuto and Jumihaidokuto, were found to inhibit the growth of <jats:italic>C.\u00a0acnes<\/jats:italic> and decrease the lipase activity. Especially, Keigairengyoto caused remarkable decrease of bacterial lipase activity. Furthermore, topical medicines such as Shiunko and Chuoko significantly decreased the lipase activity in a dose\u2010dependent manner, without inhibiting <jats:italic>C.\u00a0acnes<\/jats:italic> growth. The topical medicines were found to inhibit the expression of <jats:italic>gehA<\/jats:italic>, which codes for extracellular lipase. Our results indicate that Shiunko and Chuoko have potential as effective acne therapeutic agents, especially because they do not promote the emergence of antimicrobial\u2010resistant bacteria and skin dysbiosis.<\/jats:p>","DOI":"10.1111\/1346-8138.14810","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T06:31:00Z","timestamp":1551162660000},"page":"308-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Shiunko and Chuoko, topical Kampo medicines, inhibit the expression of <i>gehA<\/i> encoding the extracellular lipase in <i>Cutibacterium acnes<\/i>"],"prefix":"10.1111","volume":"46","author":[{"given":"Keisuke","family":"Nakase","sequence":"first","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Tashiro","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Ikoshi","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo 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