{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:20:46Z","timestamp":1747182046638,"version":"3.40.5"},"reference-count":13,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108095\u00a0h0001"],"award-info":[{"award-number":["JP19fk0108095\u00a0h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 75\u2010year\u2010old man presented with a 1\u2010cm large elastic soft subcutaneous nodule on the left side of the umbilicus, which when excised showed presence of a helminthic form within the granulomatous lesions. Morphologically, the helminth was considered to be of the genus <jats:italic>Dirofilaria<\/jats:italic>, and the patient showed increased serum antibody titer against canine filaria. The partial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequence of the mitochondrial 12S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene locus of this clinical isolate showed the highest nucleotide identity (89.6%) with <jats:italic>Dirofilaria repens<\/jats:italic>; however, the phylogenetic analysis addressed the haplotype and <jats:italic>Dirofilaria ursi<\/jats:italic> as outgroups of the clusters of <jats:italic>D<\/jats:italic>.<jats:italic>\u00a0repens<\/jats:italic> and <jats:italic>Dirofilaria immitis<\/jats:italic>, which are the causal agents of most human dirofilariasis. As like bear filaria <jats:italic>D<\/jats:italic>.<jats:italic>\u00a0ursi<\/jats:italic>, a wide variety of other carnivore\u2010parasitizing filaria species have rarely been reported in humans. The newly detected genetic haplotype in this case may correspond to one of these species of <jats:italic>Dirofilaria<\/jats:italic>, though the genetic references are not available thus far.<\/jats:p>","DOI":"10.1111\/1346-8138.15043","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T06:08:43Z","timestamp":1565158123000},"page":"914-916","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Human case of subcutaneous nodule because of a novel genetic variation of <i>Dirofilaria<\/i> sp."],"prefix":"10.1111","volume":"46","author":[{"given":"Masaharu","family":"Nakao","sequence":"first","affiliation":[{"name":"Department of Dermatology Faculty of Medicine Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa 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