{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:42:45Z","timestamp":1790458965695,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T00:00:00Z","timestamp":1566259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In patients with lepromatous leprosy, <jats:italic>Mycobacterium leprae<\/jats:italic> is often observed inside the human microvascular endothelial cells (<jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content>) surrounding Schwann cells (<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>) at the site of lesions in the peripheral nerves. Based on this observation, it is considered that the nasal mucous may be the invasion pathway for <jats:italic>M<\/jats:italic>.\u00a0<jats:italic>leprae<\/jats:italic> and <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content> serve as an important reservoir for the bacteria before they invade <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>. In light of previous research which revealed that Mce1A protein mediates bacterial invasion into nasal epithelial cells and <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content>, we conducted a study to determine whether the invasion of <jats:italic>M<\/jats:italic>.\u00a0<jats:italic>leprae<\/jats:italic> into <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content> can be suppressed by blocking the Mce1A protein. In this study, we analyzed bacterial invasive activity by adding recombinant <jats:italic>Escherichia coli<\/jats:italic>, which express the active region (InvX:72\u00a0a.a.) of Mce1A protein on their external membrane, into cultured <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content>, using the adhesin involved in the diffuse adherence mechanism. The number of bacteria that invaded into the cells was then measured by a colony counting method. The active region of Mce1A was divided into four sections, and hyperimmune antisera was prepared for each section for analyzing the inhibitory effect against invasion. The invasive activity was suppressed by antibodies against InvX regions 1\u201324\u00a0a.a., 25\u201346\u00a0a.a. and 58\u201372\u00a0a.a. This suggests that the InvX regions 1\u201324\u00a0a.a., 25\u201346\u00a0a.a. and 58\u201372\u00a0a.a. of Mce1A protein play an important role in the invasion of <jats:italic>M<\/jats:italic>.\u00a0<jats:italic>leprae<\/jats:italic> into <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content> and that it may be possible to suppress entry of <jats:italic>M<\/jats:italic>.\u00a0<jats:italic>leprae<\/jats:italic> in <jats:styled-content style=\"fixed-case\">HMVEC<\/jats:styled-content> with antibodies against these regions.<\/jats:p>","DOI":"10.1111\/1346-8138.15047","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T04:51:58Z","timestamp":1566363118000},"page":"853-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Invasion of human microvascular endothelial cells by <i>Mycobacterium leprae<\/i> through Mce1A protein"],"prefix":"10.1111","volume":"46","author":[{"given":"Irfan","family":"Idris","sequence":"first","affiliation":[{"name":"Hasanuddin University Medical Research Center Makassar Indonesia"},{"name":"Departments of Departments of Dermatology Kitasato University Graduate School of Medical Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Haykal","family":"Abdurrahman","sequence":"additional","affiliation":[{"name":"Hasanuddin University Medical Research Center Makassar Indonesia"},{"name":"Departments of Departments of Dermatology Kitasato University Graduate School of Medical Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Fatulrachman","sequence":"additional","affiliation":[{"name":"Departments of Departments of Dermatology Kitasato University Graduate School of Medical Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vienza Beby","family":"Aftitah","sequence":"additional","affiliation":[{"name":"Cellular Immunology Kitasato University Graduate School of Medical Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viesta Beby","family":"Fadlitha","sequence":"additional","affiliation":[{"name":"Departments of Departments of Dermatology Kitasato University Graduate School of Medical Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Dermatology Toshiba Rinkan Hospital Sagamihara Japan"},{"name":"Departments of Departments of Dermatology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4243-5822","authenticated-orcid":false,"given":"Takao","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Departments of Departments of Dermatology Kitasato University Graduate School of Medical Science Sagamihara Japan"},{"name":"Departments of Departments of Dermatology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Takimoto","sequence":"additional","affiliation":[{"name":"Bioscience Kitasato University School of Science Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2001.tb01308.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldl010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08053.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65414-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01335-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8367727"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.01.006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006704"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990412)81:2<305::AID-IJC22>3.0.CO;2-Z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)36030-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81927-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2076"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9857"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00142-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601775"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.15047","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1346-8138.15047","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.15047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T19:02:02Z","timestamp":1694286122000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.15047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,20]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/1346-8138.15047"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.15047","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,20]]},"assertion":[{"value":"2019-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}