{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:16Z","timestamp":1790718676355,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,9,20]],"date-time":"2019-09-20T00:00:00Z","timestamp":1568937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sato Pharmaceutical Co., Ltd."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In diagnosing onychomycosis, diseases with similar features must be excluded by demonstrating the presence of fungal infection and identifying the fungal species. However, fungal culture of onychomycosis\u2010derived samples usually takes many weeks to yield species identification results, and is associated with a low successful culture rate. Loop\u2010mediated isothermal amplification (<jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>) is a highly sensitive and specific molecular biological method that can amplify <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> at a constant temperature, allowing for a simpler testing procedure, shorter detection time and less cost than conventional techniques including quantitative polymerase chain reaction. We have developed a new <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> method specifically to detect <jats:italic>Trichophyton rubrum<\/jats:italic> (<jats:italic>T.\u00a0rubrum<\/jats:italic>) and <jats:italic>Trichophyton interdigitale<\/jats:italic> (<jats:italic>T.\u00a0interdigitale<\/jats:italic>), major causative dermatophytes for onychomycosis, and analyzed the correlation between <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> results and those of the existing fungal culture method for the detection and identification of <jats:italic>Trichophyton<\/jats:italic> species from onychomycosis\u2010derived samples. The results showed that all 59 specimens in which <jats:italic>T.\u00a0rubrum<\/jats:italic> or <jats:italic>T.\u00a0interdigitale<\/jats:italic> was identified by fungal culture also tested positive by <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>, giving a 100% positivity concordance rate between the two methods. Moreover, all 55 and four specimens in which <jats:italic>T.\u00a0rubrum<\/jats:italic> and <jats:italic>T.\u00a0interdigitale<\/jats:italic> were identified by fungal culture, respectively, also tested positive for each species by <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>, again giving a 100% species\u2010identification concordance rate. The high correlation demonstrated between <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> and fungal culture results in detection and identification of <jats:italic>Trichophyton<\/jats:italic> species from onychomycosis\u2010derived samples suggests high reliability of <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> as a promising, alternative mycological detection and identification technique which can serve as an alternative to the fungal culture method.<\/jats:p>","DOI":"10.1111\/1346-8138.15092","type":"journal-article","created":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T04:44:47Z","timestamp":1569041087000},"page":"1179-1183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Specific detection of <i>Trichophyton rubrum<\/i> and <i>Trichophyton interdigitale<\/i> based on loop\u2010mediated isothermal amplification (LAMP) from onychomycosis specimens"],"prefix":"10.1111","volume":"46","author":[{"given":"Shinichi","family":"Watanabe","sequence":"first","affiliation":[{"name":"Teikyo University Institute of Medical Mycology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7832-5639","authenticated-orcid":false,"given":"Akihiro","family":"Okubo","sequence":"additional","affiliation":[{"name":"Sato Pharmaceutical Co., Ltd. Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Miyajima","sequence":"additional","affiliation":[{"name":"Teikyo University Institute of Medical Mycology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Satoh","sequence":"additional","affiliation":[{"name":"Teikyo University Institute of Medical Mycology Tokyo Japan"},{"name":"Medical Mycology Graduate School of Medicine Teikyo University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1645-3445","authenticated-orcid":false,"given":"Koichi","family":"Makimura","sequence":"additional","affiliation":[{"name":"Teikyo University Institute of Medical Mycology Tokyo Japan"},{"name":"Medical Mycology Graduate School of Medicine Teikyo University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,20]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"851","article-title":"Diagnostic and treatment guidelines for dermatomycosis","volume":"119","author":"Watanabe S","year":"2009","journal-title":"Jpn J Dermatol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3314\/jjmm.47.11"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-016-0248-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3314\/jjmm.45.59"},{"key":"e_1_2_7_6_1","unstructured":"KamachiK AjisakaH ArakawaY.Method for detectingbordetella pertussisgene using LAMP method and primer set therefor. 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