{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:40:06Z","timestamp":1790066406856,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T00:00:00Z","timestamp":1589760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Society for Women\u2019s Health Science Research"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Use of antimicrobials for acne treatment is correlated with an increased occurrence of antimicrobial\u2010resistant <jats:italic>Cutibacterium acnes<\/jats:italic>. To clarify the role of antimicrobial use on the resistance and to investigate the characteristics of resistant strains, we conducted a multicenter study in dermatological clinics frequently visited by new patients with acne vulgaris. We collected specimens in 264 acne patients and tested 164 <jats:italic>C.\u00a0acnes<\/jats:italic> strains isolated from 164 patients visiting 13 dermatological clinics. Antimicrobial susceptibility testing showed that the rates of resistance for tetracyclines, macrolides and clindamycin were significantly higher in <jats:italic>C.\u00a0acnes<\/jats:italic> strains isolated from patients using antimicrobials for acne treatment than patients not using them. In particular, clindamycin\u2010resistant strains were frequently isolated from patients with older median age (\u226524\u00a0years) and severe\/moderate acne. After investigating the resistance mechanism of 15 high\u2010level clindamycin\u2010resistant strains, the transposable clindamycin resistance genes, <jats:italic>erm<\/jats:italic>(X) or <jats:italic>erm<\/jats:italic>(50), were detected in 14 strains. Using single\u2010locus sequence typing for <jats:italic>C.\u00a0acnes<\/jats:italic>, the strains with <jats:italic>erm<\/jats:italic>(X) or multidrug resistance plasmid pTZC1 coding <jats:italic>erm<\/jats:italic>(50) and tetracycline resistance gene <jats:italic>tet<\/jats:italic>(W) were classified into clade F, which were specifically isolated from Japanese patients with acne, except for one strain. Our data showed that patients\u2019 information, such as antimicrobial use, age and acne severity, are valuable in estimating whether a patient carries antimicrobial\u2010resistant <jats:italic>C.\u00a0acnes<\/jats:italic>. Additionally, our results suggest that the clade F strains have a high risk of acquiring multidrug resistance.<\/jats:p>","DOI":"10.1111\/1346-8138.15397","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T05:57:02Z","timestamp":1589867822000},"page":"863-869","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Characterization of acne patients carrying clindamycin\u2010resistant <i>Cutibacterium acnes<\/i>: A Japanese multicenter study"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9205-3008","authenticated-orcid":false,"given":"Keisuke","family":"Nakase","sequence":"first","affiliation":[{"name":"Department of Microbiology School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sae","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department 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